通过染料层析法从大鼠肝脏中纯化α2,6-唾液酸转移酶
Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography.
作者信息
Sticher U, Gross H J, Brossmer R
机构信息
Institut für Biochemie II, Universität Heidelberg, Federal Republic of Germany.
出版信息
Biochem J. 1988 Jul 15;253(2):577-80. doi: 10.1042/bj2530577.
Abstract
We describe a simple three-step purification for Gal-beta 1,4-GlcNAc-alpha 2,6-sialyltransferase (EC 2.4.99.1) from rat liver which uses chromatography on Cibacron Blue F3GA and f.p.l.c. It gives a highly purified (11,000-fold) enzyme in 19% yield, which is free of other sialyltransferases, CMP-NeuAc hydrolase, sialidases and proteinases.
摘要
我们描述了一种从大鼠肝脏中纯化β-1,4-半乳糖基-2,6-N-乙酰神经氨酸转移酶（EC 2.4.99.1）的简单三步法，该方法使用Cibacron Blue F3GA柱色谱和快速蛋白质液相色谱（f.p.l.c.）。该方法以19%的产率得到了高度纯化（11,000倍）的酶，且该酶不含其他唾液酸转移酶、CMP-唾液酸水解酶、唾液酸酶和蛋白酶。
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