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Separation of Gal beta 1,4GlcNAc alpha-2,6- and Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferases by affinity chromatography.

作者信息

Sabesan S, Paulson J C, Weinstein J

机构信息

Central Science and Engineering, Dupont Company, Wilmington, Delaware 19880.

出版信息

Methods Enzymol. 1994;247:237-43. doi: 10.1016/s0076-6879(94)47018-9.

DOI:10.1016/s0076-6879(94)47018-9
PMID:7898356
Abstract
摘要

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1
Separation of Gal beta 1,4GlcNAc alpha-2,6- and Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferases by affinity chromatography.
Methods Enzymol. 1994;247:237-43. doi: 10.1016/s0076-6879(94)47018-9.
2
Purification of a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase and a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase to homogeneity from rat liver.从大鼠肝脏中纯化β1,4-半乳糖基-2,6-α-唾液酸转移酶和β1,3(4)-半乳糖基-2,3-α-唾液酸转移酶至均一状态。
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5
Expression of mouse Gal beta 1,4GlcNAc alpha 2,6-sialyltransferase in an insoluble form in Escherichia coli and partial renaturation.小鼠β-1,4-N-乙酰葡糖胺α-2,6-唾液酸转移酶在大肠杆菌中的不溶性表达及部分复性
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7
Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography.通过染料层析法从大鼠肝脏中纯化α2,6-唾液酸转移酶
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Determination of the specificities of rat liver Gal(beta 1-4)GlcNAc alpha 2,6-sialyltransferase and Gal(beta 1-3/4)GlcNAc alpha 2,3-sialyltransferase using synthetic modified acceptors.
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10
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