Bas Levent, Papinski Daniel, Kraft Claudine
Max F. Perutz Laboratories, Vienna Biocenter, University of Vienna, Vienna, Austria.
Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Mol Cell Oncol. 2018 Oct 4;5(6):e1526006. doi: 10.1080/23723556.2018.1526006. eCollection 2018.
Studying the mechanism of autophagosome-vacuole fusion has proven difficult in live yeast cells. Developing a novel fusion assay, we identified Ykt6 as the missing R-SNARE (Soluble -ethylmaleimide sensitive factor attachment protein receptor) in this process and pinpoint the place of action of all four SNAREs involved. Parallel studies have confirmed our findings in other organisms.
研究自噬体与液泡融合的机制在活酵母细胞中已被证明具有挑战性。通过开发一种新的融合检测方法，我们确定Ykt6是这一过程中缺失的R-SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体），并明确了所有四种参与的SNARE的作用位点。平行研究在其他生物体中证实了我们的发现。
