Department of Chemistry and Biochemistry , The Ohio State University , 484 West 12th Avenue , Columbus , Ohio 43210 , United States.
Bioconjug Chem. 2019 Feb 20;30(2):273-283. doi: 10.1021/acs.bioconjchem.8b00778. Epub 2018 Dec 19.
Intracellular delivery of biological agents such as peptides, proteins, and nucleic acids generally rely on the endocytic pathway as the major uptake mechanism, resulting in their entrapment inside the endosome and lysosome. The recent discovery of cell-penetrating molecules of exceptionally high endosomal escape and cytosolic delivery efficiencies and elucidation of their mechanism of action represent major breakthroughs in this field. In this Topical Review, we provide an overview of the recent progress in understanding and enhancing the endosomal escape process and the new opportunities opened up by these recent findings.
细胞内递呈生物制剂（如肽、蛋白质和核酸）通常依赖内吞途径作为主要的摄取机制，导致它们被捕获在内体和溶酶体中。最近发现了具有极高的内体逃逸和细胞质递呈效率的细胞穿透分子，并阐明了它们的作用机制，这是该领域的重大突破。在这篇专题综述中，我们概述了对内体逃逸过程的理解和增强的最新进展，以及这些新发现带来的新机遇。
