Kuppner M C, Hamou M F, de Tribolet N
Neurosurgical Service, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Cancer Res. 1988 Dec 1;48(23):6926-32.
An immunohistological analysis of tumor tissue obtained from seven patients with malignant gliomas demonstrated varying levels of lymphoid cell infiltration. The tumor infiltrating lymphocytes obtained from each sample were cultured in vitro by a limiting dilution technique. In three of the cases studied many tumor infiltrating lymphocyte microcultures selectively lysed autologous glioblastoma cells but did not lyse allogeneic gliomas, natural killer-resistant fresh melanoma cells or K562 target cells. These cultures were found to consist of CD 3+ cells. In six cases studied a variable number of microcultures lysed both autologous tumor and K562 target cells only. A minority of the microcultures studied were cytolytic for allogeneic glioma cells and fresh melanoma target cells. The cytolytic activity expressed by tumor infiltrating lymphocytes against autologous tumor cells was significantly greater (P less than 0.001) than that obtained by the corresponding peripheral blood lymphocytes cultured in a similar manner. The present immunohistological and functional studies suggest that there is an immune response to human glioblastomas in vivo with an accumulation of cells with antitumor activity at the tumor site.
对7例恶性胶质瘤患者的肿瘤组织进行的免疫组织学分析显示，淋巴细胞浸润水平各不相同。通过有限稀释技术在体外培养从每个样本中获取的肿瘤浸润淋巴细胞。在所研究的3例病例中，许多肿瘤浸润淋巴细胞微培养物选择性地裂解自体胶质母细胞瘤细胞，但不裂解同种异体胶质瘤、天然杀伤抗性新鲜黑色素瘤细胞或K562靶细胞。发现这些培养物由CD 3+细胞组成。在所研究的6例病例中，数量不等的微培养物仅裂解自体肿瘤和K562靶细胞。所研究的少数微培养物对同种异体胶质瘤细胞和新鲜黑色素瘤靶细胞具有细胞溶解活性。肿瘤浸润淋巴细胞对自体肿瘤细胞表达的细胞溶解活性明显高于（P小于0.001）以类似方式培养的相应外周血淋巴细胞所获得的活性。目前的免疫组织学和功能研究表明，体内对人胶质母细胞瘤存在免疫反应，肿瘤部位有具有抗肿瘤活性的细胞聚集。
