Center for Neuroscience, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong, 515041, People's Republic of China.
Center for Neuroscience, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong, 515041, People's Republic of China.
Brain Res Bull. 2019 Jan;144:180-186. doi: 10.1016/j.brainresbull.2018.12.002. Epub 2018 Dec 7.
Accumulated evidence has recently demonstrated that spinal cord injury (SCI) can lead to chronic damage in a wide range of brain regions. Neuregulin 1 (Nrg1) signaling has been broadly recognized as an important mechanism contributing to neural differentiation and regeneration. We here studied the effect of SCI on Nrg1 signaling in prefrontal cortex (PFC) and hippocampus (HIP) in a mouse model. As was indicated by the increased levels of GFAP and Iba-1, our results demonstrated that SCI significantly induced activation of astrocytes and microglial cells in both PFC and HIP. In addition, both western blot and morphological assay demonstrated that Nrg1 was altered in both regions at 8 weeks post SCI, which was accompanied with decreased phosphorylation levels of its cognitive receptors Neu and ErbB4. Our combined results indicated that SCI can influence Nrg1 signaling, which may contribute to the worsening of pathophysiological changes in major brain regions during SCI. These results also suggested that exogenous Nrg1 treatment may have a therapeutic role in counteracting SCI-induced brain damage.
最近的研究证据表明，脊髓损伤（SCI）会导致广泛的大脑区域的慢性损伤。神经调节蛋白 1（Nrg1）信号已被广泛认为是促进神经分化和再生的重要机制。我们在此研究了 SCI 对小鼠模型前额叶皮层（PFC）和海马（HIP）中 Nrg1 信号的影响。我们的结果表明，GFAP 和 Iba-1 水平的增加表明，SCI 显著诱导了 PFC 和 HIP 中星形胶质细胞和小胶质细胞的激活。此外，Western blot 和形态学分析均表明，SCI 后 8 周，两个区域的 Nrg1 发生改变，其认知受体 Neu 和 ErbB4 的磷酸化水平降低。我们的综合结果表明，SCI 可以影响 Nrg1 信号，这可能导致 SCI 期间主要脑区的病理生理变化恶化。这些结果还表明，外源性 Nrg1 治疗可能在对抗 SCI 引起的脑损伤方面具有治疗作用。
