da Cunha Jessyka Arruda, Bandeira Junior Guerino, da Silva Elisia Gomes, de Ávila Scheeren Cecília, Fausto Viviane Pedroso, Salbego Joseânia, de Almeida Vaucher Rodrigo, de Vargas Agueda Castagna, Baldisserotto Bernardo
Graduate Program in Pharmacology, Universidade Federal de Santa Maria/ UFSM, 97105-900, Santa Maria, RS, Brazil.
Pharmacy Course, UFSM, Santa Maria, RS, Brazil.
Microb Pathog. 2019 Feb;127:220-224. doi: 10.1016/j.micpath.2018.12.005. Epub 2018 Dec 5.
The aim of this study was to evaluate the resistance of Rhamdia quelen (silver catfish) to Aeromonas hydrophila infection after treatment with pure and nanoencapsulated forms either terpinen-4-ol, thymol, or carvacrol and the effects of these treatments on fish metabolic responses. After A. hydrophila inoculation, fish were treated with 30 min daily baths for 6 consecutive days with terpinen-4-ol, thymol, or carvacrol in their pure or nanoencapsulated forms at concentrations of 5, 10, 15 or 25 mg L. A positive control group, negative control group and saline group were also included. Survival was evaluated at the end of treatment for six consecutive days. Muscle and liver were collected to determine glucose and lactate levels. The fish treated with the nanoencapsulated form of the compounds had a high survival rate, similar to saline group and negative control groups. The carvacrol, thymol and terpinen-4-ol nanoencapsulated forms improved survival of silver catfish infected with A. hydrophila. Muscle and liver glucose and lactate levels are not indicated as biomarkers because they did not present any correlation between the metabolic state of the fish and the bacterial infection.
本研究的目的是评估用萜品烯 -4-醇、百里香酚或香芹酚的纯品和纳米包封形式处理后，奎氏滑鲇（银鲶鱼）对嗜水气单胞菌感染的抵抗力，以及这些处理对鱼类代谢反应的影响。在接种嗜水气单胞菌后，鱼连续6天每天用萜品烯 -4-醇、百里香酚或香芹酚的纯品或纳米包封形式进行30分钟浸浴处理，浓度分别为5、10、15或25 mg/L。还包括一个阳性对照组、一个阴性对照组和一个生理盐水组。在连续6天的处理结束时评估存活率。采集肌肉和肝脏以测定葡萄糖和乳酸水平。用化合物纳米包封形式处理的鱼具有较高的存活率，与生理盐水组和阴性对照组相似。香芹酚、百里香酚和萜品烯 -4-醇的纳米包封形式提高了感染嗜水气单胞菌的银鲶鱼的存活率。肌肉和肝脏中的葡萄糖和乳酸水平未被表明为生物标志物，因为它们在鱼类的代谢状态和细菌感染之间未呈现任何相关性。
