O'Halloran Damien M
Department of Biological Sciences, George Washington University, Science and Engineering Hall, Suite 6000, 800 22nd Street NW, Washington DC 20052, USA; Institute for Neuroscience, George Washington University, 636A Ross Hall, 2300 I Street NW, Washington DC 20052, USA.
Mol Biochem Parasitol. 2019 Jan;227:25-28. doi: 10.1016/j.molbiopara.2018.12.001. Epub 2018 Dec 7.
The pace of research towards a genetic model to understand the unique molecular biology of parasitic nematodes has increased recently. This research has developed a diverse suite of genetic tools for a variety of parasitic nematodes. CRISPR/Cas9 technology in particular offers much promise as a game changing tool for researchers studying parasitic nematodes. Unlike RNAi, which depends on diverse nematode effectors to silence gene expression, the effectors for CRISPR/Cas9 mutations are typically supplied by the experimenter, making gene editing via CRISPR/Cas9 ideal for testing on genetically intractable nematode systems. To facilitate the development of CRISPR/Cas9 technology for parasitic nematodes, I here describe a tool for identifying gRNA targets and diagnostic primers to a user supplied sequence. The software attempts to minimize non-specific targets by interrogating the genomes of parasitic nematodes. This software is freely available online and features an intuitive interface to help researchers design effective CRISPR experiments for parasitic nematodes.
最近，为理解寄生线虫独特分子生物学而构建遗传模型的研究步伐加快了。这项研究为多种寄生线虫开发了一系列多样的遗传工具。尤其是CRISPR/Cas9技术，作为一种改变游戏规则的工具，为研究寄生线虫的科研人员带来了很大希望。与依赖多种线虫效应物来沉默基因表达的RNA干扰不同，CRISPR/Cas9突变的效应物通常由实验者提供，这使得通过CRISPR/Cas9进行基因编辑非常适合在遗传上难以处理的线虫系统上进行测试。为促进用于寄生线虫的CRISPR/Cas9技术的发展，我在此描述一种工具，用于识别向导RNA（gRNA）靶点和针对用户提供序列的诊断引物。该软件试图通过查询寄生线虫的基因组来尽量减少非特异性靶点。此软件可在网上免费获取，其界面直观，有助于研究人员为寄生线虫设计有效的CRISPR实验。
