McClanahan Patrick, Le Tuan Anh, Cockx Bram, Temmerman Liesbet
KU Leuven, Leuven, Flanders, Belgium.
MicroPubl Biol. 2023 Apr 26;2023. doi: 10.17912/micropub.biology.000808. eCollection 2023.
Cryopreservation allows strains to be stored, eliminating genetic drift and maintenance costs. Existing cryopreservation methods for the economically-important entomopathogenic nematode involve multiple incubation and filtration steps to precondition the animals. The standard protocol for freezing the model organism in buffer is simpler, and a recent dry-freezing protocol allows stocks to survive multiple freeze-thaws, a possibility during a power failure. Here we report the efficacy of cryopreservation protocols adapted for . We show that dry freezing with disaccharides, but not glycerol-based or trehalose-DMSO-based freezing buffer, allows reliable recovery of infective juveniles.
冷冻保存可使菌株得以储存，消除基因漂移并降低维护成本。针对具有经济重要性的昆虫病原线虫，现有的冷冻保存方法需要多个孵育和过滤步骤来预处理线虫。在缓冲液中冷冻模式生物的标准方案更为简单，并且最近的一种干冻方案能使菌液在多次冻融后仍存活，这在停电期间是有可能发生的情况。在此，我们报告了适用于[具体对象未给出]的冷冻保存方案的效果。我们发现，使用二糖进行干冻可使感染性幼虫可靠复苏，而基于甘油或海藻糖 - 二甲基亚砜的冷冻缓冲液则不行。
