Yanagisawa H, Manabe S, Kanai Y, Wada O
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Clin Nephrol. 1988 Aug;30(2):73-8.
By using a high-performance liquid chromatography (HPLC) method, we determined the contents of 2-amino-6-methyldipyrido[1,2-a:3', 2'-d] imidazole (Glu-P-1) and 2-aminodipyrido[1,2-a:3', 2'-d]imidazole (Glu-P-2) in the dialysate of patients with uremia. The total dialysate (approximately 6 liters) per day of the patients who had received continuous ambulatory peritoneal dialysis (CAPD) was examined. The amounts of Glu-P-1 and Glu-P-2 in the total dialysate per day were 552.2 +/- 266.9 pmole and 386.3 +/- 146.0 pmole (mean +/- SD, n = 10), respectively. The recoveries of Glu-P-1 and Glu-P-2 in the dialysate on our assay method were 68.2% and 54.3%, respectively. Based on the recoveries, the average amounts of Glu-P-1 and Glu-P-2 in the dialysate of uremic patients were assumed to be 809.7 pmole and 711.4 pmole, respectively.
通过使用高效液相色谱（HPLC）方法，我们测定了尿毒症患者透析液中2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1）和2-氨基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-2）的含量。对接受持续性非卧床腹膜透析（CAPD）的患者每天的总透析液（约6升）进行了检测。每天总透析液中Glu-P-1和Glu-P-2的含量分别为552.2±266.9皮摩尔和386.3±146.0皮摩尔（平均值±标准差，n = 10）。我们的检测方法中透析液中Glu-P-1和Glu-P-2的回收率分别为68.2%和54.3%。基于这些回收率，推测尿毒症患者透析液中Glu-P-1和Glu-P-2的平均含量分别为809.7皮摩尔和711.4皮摩尔。
