Ogawa H, Saito A, Oda O, Nakajima M, Chung T G
Department of Internal Medicine, Shinseikai Dai-Ichi Hospital, Nagoya, Japan.
Clin Nephrol. 1988 Sep;30(3):158-63.
Serum from 8 undialyzed patients and 30 dialyzed patients was examined by immunoblotting using anti beta 2-microglobulin (beta 2M) serum after two-dimensional gel electrophoresis (2.DE). One major spot and three minor spots were detected in the ultrafiltrate as well as in the serum. One major spot was determined to be native beta 2M and three minor spots were found to be novel beta 2M. Novel beta 2M had a lower molecular weight (MW) and a higher acidic isoelectric point (pI). Novel beta 2M was recognized in the sera of 5 out of 20 hemodialysis (HD) patients without carpal tunnel syndrome (CTS), 2 of whom had been on HD from 5 to 10 years and 3 for more than 10 years, as well as in the sera of all 10 patients with CTS. By chromatofocusing, pI of novel beta 2M was 5.2, while pI of native beta 2M was 5.7. When the tissue specimen of transverse carpal ligament of 2 HD patients with CTS was examined by immunoblotting after 2.DE, the spot of novel beta 2M was larger than that of native beta 2M. It is possible that some metabolic abnormality of beta 2M occurs through long-term hemodialysis, and it is possible that novel beta 2M might relate to amyloidogenic predisposition.
采用抗β2-微球蛋白（β2M）血清，经二维凝胶电泳（2-DE）后，通过免疫印迹法检测了8例未透析患者和30例透析患者的血清。在超滤液以及血清中检测到一个主要斑点和三个次要斑点。确定一个主要斑点为天然β2M，三个次要斑点为新型β2M。新型β2M的分子量（MW）较低，酸性等电点（pI）较高。在20例无腕管综合征（CTS）的血液透析（HD）患者中，有5例患者的血清中检测到新型β2M，其中2例接受血液透析5至10年，3例超过10年，并且在所有10例CTS患者的血清中也检测到新型β2M。通过色谱聚焦法，新型β2M的pI为5.2，而天然β2M的pI为5.7。当对2例患有CTS的HD患者的腕横韧带组织标本进行2-DE后免疫印迹检测时，新型β2M的斑点比天然β2M的斑点大。长期血液透析可能导致β2M发生一些代谢异常，新型β2M可能与淀粉样变性易感性有关。
