University of British Columbia, Michael Smith Laboratories, 2329 West Mall, Vancouver, BC V6T 1Z4, Canada.
University of British Columbia, Michael Smith Laboratories, 2329 West Mall, Vancouver, BC V6T 1Z4, Canada.
Curr Opin Chem Biol. 2019 Feb;48:81-85. doi: 10.1016/j.cbpa.2018.11.003. Epub 2018 Dec 4.
Identifying protein-protein interactions (PPIs) is necessary to understand the molecular mechanisms behind cellular processes. This task is complicated by the facts that many proteins can interact simultaneously (i.e. a protein complex) and may participate in more than one distinct complex. Because of this, a large number of combinatorial arrangements are possible, both of PPIs and complexes, making it a difficult task to identify all truly interacting proteins. Protein interactions also range from stable to highly transient assemblies, with lifetimes on the order of seconds [1]. Therefore, studies identifying PPIs must not only contend with the arrangement of proteins into PPIs and complexes, but the stability of the interactions as well. Because of the difficulty of the task, many approaches have been used to identify and study the dynamics of PPIs. In this review, we will summarize a number of the techniques currently used to identify protein-protein interactions, with a focus on recent developments.
鉴定蛋白质-蛋白质相互作用（PPIs）对于理解细胞过程背后的分子机制是必要的。然而，许多蛋白质可以同时相互作用（即蛋白质复合物），并且可能参与不止一个独特的复合物，这使得这项任务变得复杂。由于这个原因，可能存在大量的 PPI 和复合物的组合排列，使得鉴定所有真正相互作用的蛋白质成为一项艰巨的任务。蛋白质相互作用的稳定性也从稳定到高度瞬态组装不等，其寿命在几秒钟的范围内[1]。因此，鉴定蛋白质相互作用的研究不仅必须应对蛋白质排列成 PPI 和复合物的问题，还必须应对相互作用的稳定性问题。由于任务的难度，已经使用了许多方法来鉴定和研究 PPI 的动态。在这篇综述中，我们将总结目前用于鉴定蛋白质-蛋白质相互作用的一些技术，重点介绍最近的发展。
