School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032, Camerino, MC, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032, Camerino, MC, Italy.
Environ Pollut. 2019 Mar;246:99-106. doi: 10.1016/j.envpol.2018.11.098. Epub 2018 Nov 30.
There is evidence that polycyclic aromatic hydrocarbons (PAHs) are consistently the predominant organic contaminants in concentration found in loggerhead sea turtles (Caretta caretta) from the North and Central Adriatic Sea. Hence this study investigates the PAH toxicity to loggerheads by using a particular set of genes [i.e. CYP1B, CAT, GPX, GSTT1, SOD3, DNMT1, Epoxide hydrolase 1 (EPHX1), Poly (ADP-ribose) polymerase 1 (PARP1), Lamin-A/C isoform 3 (LMNA), Talin 1 (TLN1), Annexin A1 (ANXA1)] whose altered expression is potentially dependent on and specific for the PAH-related mechanism of action. Twenty healthy juvenile loggerheads were thus divided into high and low exposure groups (mean of ΣPAHs: 80.34 ng mL vs. 8.84 ng mL, P < 0.0001) according to the median split of ΣPAHs. Interestingly, we found that the whole blood mRNA levels of each gene biomarker tested were significantly increased in high PAH-exposed turtles thus proving to be useful for the biological monitoring of PAH toxicity and hematotoxicity in sea turtles.
有证据表明，多环芳烃（PAHs）是北亚得里亚海和中亚得里亚海的红海龟（Caretta caretta）体内浓度最高的有机污染物。因此，本研究通过使用一组特定的基因[即 CYP1B、CAT、GPX、GSTT1、SOD3、DNMT1、环氧化物水解酶 1（EPHX1）、多聚（ADP-核糖）聚合酶 1（PARP1）、核纤层蛋白-A/C 异构体 3（LMNA）、桩蛋白 1（TLN1）、膜联蛋白 A1（ANXA1）]来研究 PAH 对红海龟的毒性，这些基因的表达可能与 PAH 相关的作用机制有关，且具有特异性。因此，根据 ΣPAHs 的中位数将 20 只健康的幼年红海龟分为高暴露组和低暴露组（ΣPAHs 的平均值分别为 80.34ng/mL 和 8.84ng/mL，P<0.0001）。有趣的是，我们发现，高 PAH 暴露组海龟的全血 mRNA 水平均显著升高，表明这些基因生物标志物可用于生物监测 PAH 毒性和海龟的血液毒性。
