Egypt-Japan University of Science and Technology (E-JUST), Environmental Engineering Department, P.O. Box 179, New Borg Al Arab City, Alexandria, 21934, Egypt; Tokyo Institute of Technology, Civil and Environmental Engineering Department, Meguro-ku, Tokyo, 152-8552, Japan; Zagazig University, Environmental Engineering Department, Zagazig, 44519, Egypt.
Tanta University, Faculty of Engineering, Public Works Engineering Department, 31521, Tanta City, Egypt.
J Environ Manage. 2019 Feb 15;232:875-886. doi: 10.1016/j.jenvman.2018.11.134. Epub 2018 Dec 6.
In this study, compartment-wise investigation of an anammox baffled reactor (AnBR) was performed. The AnBR achieved steady-state conditions after a start-up period of ∼50 days and achieved NH and NO conversion percentages of 88.5 and 99.3%, respectively. Examination of the nitrogen mass balance revealed that an AnBR with a two-compartment configuration was sufficient for nitrogen loading rates (NLRs) ranging from 0.125 to 1.975 kg N/m/d and resulted in a nitrogen removal efficiency (NRE) of 86.7-93.7%. Higher NLRs (4.04-5.05 kg N/m/d) required four compartments to achieve an NRE of 82.2-87.1%. Further, an overall NLR increase of up to 5.93 ± 0.23 kg N/m/d resulted in complete AnBR failure. The maximum nitrogen removal rate was consistently recorded in the 1 compartment for all NLRs examined; as a result, this compartment exhibited the highest bacterial activity. Biomass concentration, specific anammox activity, extracellular polymeric substances, and average granule diameter in the 1 compartment with an overall NLR of 0.05 kg N/m/d were estimated to be 11.2 g/L, 0.03 mg N/g/h, 84.3 mg/g and 0.65 mm, respectively. These values increased to 26.1 g/L, 11.80 mg N/g/h, 242.1 mg/g and 2.31 mm, respectively, when the overall NLR was incremented to 4.04 kg N/m/d. However, a gradual reduction in bacterial activity was observed from the 1 to the 5 compartment. The microbial community analysis indicated that the dominant phyla in the 1 compartment (NLR of 0.252 kg N/m/d) with the highest nitrogen removal were Chloroflexi (38.13%), Planctomycetes (22.62%), and Proteobacteria (14.75%).
在这项研究中，对 anammox 折流反应器 (AnBR) 进行了分区研究。AnBR 在启动期约 50 天后达到稳定状态，分别实现了 88.5%和 99.3%的 NH 和 NO 转化率。氮质量平衡检验表明，具有双隔室配置的 AnBR 足以适应氮负荷率 (NLR) 范围为 0.125 至 1.975 kg N/m/d，并实现了 86.7-93.7%的氮去除效率 (NRE)。较高的 NLR (4.04-5.05 kg N/m/d) 需要四个隔室才能达到 82.2-87.1%的 NRE。此外，NLR 总体增加高达 5.93±0.23 kg N/m/d 导致 AnBR 完全失效。在所有检查的 NLR 中，最大的氮去除率始终记录在 1 隔室中；因此，该隔室表现出最高的细菌活性。在 NLR 总体为 0.05 kg N/m/d 的 1 隔室中估计的生物量浓度、特定的 anammox 活性、胞外聚合物物质和平均颗粒直径分别为 11.2 g/L、0.03 mg N/g/h、84.3 mg/g 和 0.65 mm。当 NLR 总体增加到 4.04 kg N/m/d 时，这些值分别增加到 26.1 g/L、11.80 mg N/g/h、242.1 mg/g 和 2.31 mm。然而，从第 1 隔室到第 5 隔室，细菌活性逐渐降低。微生物群落分析表明，在氮去除率最高的 1 隔室（NLR 为 0.252 kg N/m/d）中占主导地位的菌群是绿弯菌门 (Chloroflexi) (38.13%)、浮霉菌门 (Planctomycetes) (22.62%) 和变形菌门 (Proteobacteria) (14.75%)。
