Simon Claire S, Zhang Lu, Wu Tao, Cai Weibin, Saiz Nestor, Nowotschin Sonja, Cai Chen-Leng, Hadjantonakis Anna-Katerina
Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Department of Developmental and Regenerative Biology, The Mindich Child Health and Development Institute, and The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Biol Open. 2018 Dec 10;7(12):bio036517. doi: 10.1242/bio.036517.
The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and in adult organs. These include the primitive endoderm of the blastocyst, visceral endoderm of the early post-implantation embryo, as well as lateral plate mesoderm, developing heart, liver, lung and gonads. Here, we generate a novel targeted allele used to generate both a transcriptional reporter and a fusion protein to analyse dynamic expression domains. We demonstrate that the transcriptional reporter faithfully recapitulates known sites of mRNA expression and correlates with endogenous GATA4 protein levels. This reporter labels nuclei of expressing cells and is suitable for time-lapse imaging and single cell analyses. As such, this allele will be a useful tool for studying expression and transcriptional regulation.This article has an associated First Person interview with the first author of the paper.
GATA锌指转录因子GATA4在小鼠胚胎发育过程中的多种组织以及成体器官中均有表达。这些组织包括囊胚的原始内胚层、植入后早期胚胎的脏内胚层，以及侧板中胚层、发育中的心脏、肝脏、肺和性腺。在此，我们构建了一个新型靶向等位基因，用于生成转录报告基因和融合蛋白，以分析动态表达结构域。我们证明，该转录报告基因忠实地重现了已知的mRNA表达位点，并与内源性GATA4蛋白水平相关。该报告基因标记表达细胞的细胞核，适用于延时成像和单细胞分析。因此，这个等位基因将成为研究表达和转录调控的有用工具。本文配有对该论文第一作者的第一人称访谈。
