18F-FDG-PET 的代谢-容积学参数可预测子宫颈癌中 EGFR 和 HIF 1alpha 的表达。

Metabolo-volumetric parameters of 18F-FDG-PET can predict expression of EGFR and HIF 1alpha in uterine cervical cancer.

机构信息

Department of Diagnostic and Interventional Radiology, University Hospital of Leipzig, Leipzig 04103, Germany.

Department of Pathology, University Hospital of Leipzig, Leipzig 04103, Germany.

出版信息

Cancer Biomark. 2019;24(1):135-140. doi: 10.3233/CBM-182019.

DOI:10.3233/CBM-182019

PMID:30530968

Abstract

BACKGROUND

The aim of our study was to investigate possible relationships between 18F-FDG-PET parameters and clinically relevant histopathological findings in patients with cervical cancer (CC).

METHODS

Eighteen female patients (mean age 55.4 years) with histologically confirmed squamous cell CC were involved into the study. In all cases, 18F-FDG-PET CT was performed. Mean and maximum standardized uptake values (SUVmean and SUVmax), total lesion glycolysis (TLG) and metabolic tumor volume (MTV) were determined on PET-images. For every tumor the following specimen stainings were performed: epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), tumor suppressor protein p53, hypoxia-inducible factor (HIF)-1α, and histone 3. All stained specimens were digitalized and analyzed by using the ImageJ software 1.48v. Spearman's correlation coefficient (p) was used to analyze associations between investigated parameters. p-values < 0.05 were taken to indicate statistical significance.

RESULTS

TLG and MTV correlated well with expression of EGFR (p= 0.601, P= 0.008 and p= 0.586, P= 0.011, respectively). SUVmedian correlated inversely with expression of HIF 1alpha (p=-0.509, P= 0.031). SUVmean tended to correlate with expression of EGFR and HIF 1alpha. None of the PET parameters correlated with expression of Histone 3, p53 and VEGF.

CONCLUSION

TLG and MTV can reflect expression of EGFR and SUVmedian correlated significantly with expression of HIF-1α. None of the PET parameters can predict expression of Histone 3, p53 and VEGF.

摘要

背景

本研究旨在探讨宫颈癌（CC）患者中 18F-FDG-PET 参数与临床相关组织病理学发现之间的可能关系。

方法

18 名经组织学证实患有鳞状细胞 CC 的女性患者（平均年龄 55.4 岁）参与了本研究。所有患者均进行了 18F-FDG-PET CT 检查。在 PET 图像上确定平均和最大标准化摄取值（SUVmean 和 SUVmax）、总病变糖酵解（TLG）和代谢肿瘤体积（MTV）。对每个肿瘤进行以下标本染色：表皮生长因子受体（EGFR）、血管内皮生长因子（VEGF）、肿瘤抑制蛋白 p53、缺氧诱导因子（HIF）-1α和组蛋白 3。所有染色标本均使用 ImageJ 软件 1.48v 进行数字化和分析。使用 Spearman 相关系数（p）分析研究参数之间的关联。p 值<0.05 表示具有统计学意义。

结果

TLG 和 MTV 与 EGFR 的表达密切相关（p=0.601，P=0.008 和 p=0.586，P=0.011）。SUVmedian 与 HIF 1alpha 的表达呈负相关（p=-0.509，P=0.031）。SUVmean 与 EGFR 和 HIF 1alpha 的表达呈正相关趋势。PET 参数与组蛋白 3、p53 和 VEGF 的表达均无相关性。