Chen H T, Pittman C S
Division of Endocrinology and Metabolism, University of Alabama, Birmingham 35294.
Mol Cell Endocrinol. 1988 Oct;59(3):233-40. doi: 10.1016/0303-7207(88)90108-6.
The long-term in vivo effect of diethylstilbestrol (DES) on the expression of a cell-surface antigen associated with the anterior pituitary somatotroph was studied in two strains of female rats using double immunofluorescence techniques. Mab WHC-1, a recently generated and characterized monoclonal antibody, was used to detect the antigen associated with somatotrophs, whereas rabbit anti-rat prolactin (rPRL) and anti-human growth hormone (hGH) antisera were used to identify mammotrophs and somatotrophs, respectively. In F344 rats, Mab WHC-1-positive cells increased from 13.8 +/- 0.5% of total pituitary cells in normal anterior pituitaries to 34.2 +/- 4.0% in DES-induced pituitary tumors. The number of mammotrophs also increased significantly from 58.0 +/- 3.2% in controls to 75.9 +/- 2.2% in tumors. On the other hand, somatotrophs decreased significantly in number following ovariectomy (OVX) and DES implantation (19.7 +/- 0.5% vs. 6.1 +/- 1.2%). Based on double immunofluorescence, the percentage of Mab WHC-1-positive cells, which were somatotrophs, decreased from 85.5 +/- 2.7% in normal controls to 6.7 +/- 1.5% in DES-induced tumors. On the other hand, the percentage of Mab WHC-1-positive cells which were mammotrophs increased significantly from 14.0 +/- 1.4% to 86.1 +/- 1.8% following OVX and DES implantation. A similar change was found in the number of somatotrophs and mammotrophs following the same treatment in Sprague-Dawley (SD) rats which did not develop pituitary tumors. In contrast to F344 rats, the number of Mab WHC-1-positive cells in SD rats decreased significantly from 32.4 +/- 2.8% in sham-operated controls to 19.3 +/- 2.9% in OVX + DES-implanted rats.(ABSTRACT TRUNCATED AT 250 WORDS)
采用双重免疫荧光技术，在两个品系的雌性大鼠中研究了己烯雌酚（DES）对与垂体前叶生长激素细胞相关的细胞表面抗原表达的长期体内效应。使用最近产生并鉴定的单克隆抗体Mab WHC-1来检测与生长激素细胞相关的抗原，而兔抗大鼠催乳素（rPRL）和抗人生长激素（hGH）抗血清分别用于鉴定泌乳激素细胞和生长激素细胞。在F344大鼠中，Mab WHC-1阳性细胞从正常垂体前叶中占垂体细胞总数的13.8±0.5%增加到DES诱导的垂体肿瘤中的34.2±4.0%。泌乳激素细胞的数量也从对照组的58.0±3.2%显著增加到肿瘤组的75.9±2.2%。另一方面，卵巢切除（OVX）和DES植入后，生长激素细胞的数量显著减少（19.7±0.5%对6.1±1.2%）。基于双重免疫荧光，作为生长激素细胞的Mab WHC-1阳性细胞的百分比从正常对照组的85.5±2.7%降至DES诱导肿瘤中的6.7±1.5%。另一方面，作为泌乳激素细胞的Mab WHC-1阳性细胞的百分比在OVX和DES植入后从14.0±1.4%显著增加到86.1±1.8%。在未发生垂体肿瘤的Sprague-Dawley（SD）大鼠中，相同处理后生长激素细胞和泌乳激素细胞的数量也出现了类似变化。与F344大鼠不同，SD大鼠中Mab WHC-1阳性细胞的数量从假手术对照组的32.4±2.8%显著降至OVX + DES植入大鼠的19.3±2.9%。（摘要截短于250字）
