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大肠杆菌NGF-1,一种具有遗传可操作性、能有效定殖于小鼠肠道的分离菌株。

Escherichia coli NGF-1, a Genetically Tractable, Efficiently Colonizing Murine Gut Isolate.

作者信息

Ziesack Marika, Karrenbelt Michiel A P, Bues Johannes, Schaefer Elena, Silver Pamela, Way Jeffrey

机构信息

Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA.

Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.

出版信息

Microbiol Resour Announc. 2018 Dec 6;7(22). doi: 10.1128/MRA.01416-18. eCollection 2018 Dec.

DOI:10.1128/MRA.01416-18
PMID:30533859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6284091/
Abstract

The genome of the murine commensal strain Escherichia coli NGF-1 contains a 5.03-Mbp chromosome and plasmids of 40.2 kbp and 8.56 kbp. NGF-1 efficiently colonizes the mouse gut and is genetically tractable. The genome sequence reported here facilitates genetic engineering and research in mouse models of healthy and diseased intestine.

摘要

小鼠共生菌株大肠杆菌NGF-1的基因组包含一条5.03兆碱基对的染色体以及40.2千碱基对和8.56千碱基对的质粒。NGF-1能有效地定殖于小鼠肠道,并且在遗传学上易于操作。此处报道的基因组序列有助于在健康和患病肠道的小鼠模型中进行基因工程和研究。