Oladeinde Bankole H, Ekejindu Ifeoma M, Omoregie Richard, Odia Ikpomwonosa, Aguh Obiora D, Okwu Uchechukwu M
Department of Medical Laboratory Science, Edo University, Iyamho, Edo State, Nigeria.
Department of Medical Laboratory Sciences, Faculty of Health Sciences, College of Health Science, Nnamdi Azikiwe University, Awka, Nigeria.
Int J Health Sci (Qassim). 2018 Nov-Dec;12(6):25-29.
Hepatitis B virus (HBV) is not uncommon among persons infected with human immunodeficiency virus (HIV). Severity of HBV infection and treatment outcome are associated with specific HBV genotypes. No study has reported the types of HBV genotypes circulating among HIV-infected subjects in Nigeria. This study was designed to determine the prevalence of HBV, as well as its genotypic distribution among HIV-infected subjects in Benin City, Nigeria.
Whole blood was collected from a total of 564 HIV-infected and 250 apparently healthy HIV-negative subjects. Serodiagnosis of HBV infection was done using an immunochromatographic kit. Detection of HBV-DNA and sequencing of amplicons were done using standard molecular techniques.
HIV status was not significantly associated with HBV seroinfection (HIV vs. non-HIV: 4.6% vs. 4.0%; odds ratio = 1.168, 95% confidence interval = 0.550, 2.444, and P = 0.854). HIV-infected subjects were observed to have an insignificantly (P = 0.645) higher prevalence of true HBV infection than their non-HIV-infected counterparts (HIV positive vs. HIV negative: 23.1% vs. 10.0%). All patients with true HBV infection were found to harbor HBV genotype E, which did not cluster around other HBV genotype E.
This study reports novel strains of HBV genotype E circulating in Nigeria.
乙肝病毒（HBV）在感染人类免疫缺陷病毒（HIV）的人群中并不罕见。HBV感染的严重程度和治疗结果与特定的HBV基因型有关。尚无研究报道尼日利亚HIV感染受试者中流行的HBV基因型类型。本研究旨在确定尼日利亚贝宁城HIV感染受试者中HBV的流行率及其基因型分布。
共采集了564例HIV感染受试者和250例表面健康的HIV阴性受试者的全血。使用免疫层析试剂盒对HBV感染进行血清学诊断。采用标准分子技术进行HBV-DNA检测和扩增子测序。
HIV状态与HBV血清感染无显著相关性（HIV感染者与非HIV感染者：4.6%对4.0%；优势比=1.168，95%置信区间=0.550，2.444，P=0.854）。观察发现，HIV感染受试者的真正HBV感染患病率略高于未感染HIV的受试者（HIV阳性对HIV阴性：23.1%对10.0%，P=0.645）。所有真正感染HBV的患者均携带HBV基因型E，该基因型未与其他HBV基因型E聚类。
本研究报告了在尼日利亚流行的新型HBV基因型E毒株。
