Liu Wen, Shen Xiangling, Liang Hongwei, Wang Yubing, He Zhengquan, Zhang Dechun, Chen Faju
Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, Yichang, China.
Front Plant Sci. 2018 Nov 26;9:1743. doi: 10.3389/fpls.2018.01743. eCollection 2018.
() homologs are crucial regulators of flower development in angiosperms. In this study, we isolated the homolog from , a basal angiosperm belonging to the Magnoliaceae. Molecular phylogenetic analysis suggested that was grouped into the PI/GLO lineages of B-class MADS-box gene with the distinctive PI motif. Further expression profiling analysis showed that was expressed in tepals and stamens but not in juvenile leaves and carpels, similar to the spatial expression pattern of in Arabidopsis. Interestingly, had higher expression level in inner-tepals than in outer-tepals, whereas the flower is homochlamydeous. Moreover, ectopic expression of in Arabidopsis mutant emerged filament-like structures but had no obvious petals, suggesting a partial phenotypic recovery of mutant. The features of in the expression pattern and gene function improved our acknowledgment of B-class genes in , and contributed to the clarification of evolution status and relations with other sibling species in molecular perspective.
（）同源物是被子植物花发育的关键调节因子。在本研究中，我们从木兰科的一种基部被子植物中分离出了该同源物。分子系统发育分析表明，该同源物与具有独特PI基序的B类MADS-box基因的PI/GLO谱系归为一类。进一步的表达谱分析表明，该同源物在花被片和雄蕊中表达，但在幼叶和心皮中不表达，这与拟南芥中该基因的空间表达模式相似。有趣的是，该同源物在内层花被片中的表达水平高于外层花被片，而该植物的花是同被花。此外，该同源物在拟南芥突变体中的异位表达出现了丝状结构，但没有明显的花瓣，这表明突变体的部分表型得到了恢复。该同源物在表达模式和基因功能方面的特征增进了我们对该植物中B类基因的认识，并有助于从分子角度阐明该植物的进化地位及其与其他近缘物种的关系。
