Bokoliya Suresh, Patil Shripad, Nagappa Madhu, Taly Arun
Department of Neuromicrobiology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, India.
Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, India.
Lab Med. 2019 Jul 16;50(3):229-235. doi: 10.1093/labmed/lmy038.
To assess the practicality of dot-blot testing for rapid and sensitive detection of the antiacetylcholine receptor (anti-AChR) antibodies in myasthenia gravis (MG).
In this case-control study, we tested serum specimens of 85 patients with MG, 85 healthy control individuals, and 85 patients without MG who have other autoimmune and neurological illnesses. All the serum specimens were tested for anti-AChR antibodies using 3 assays: in-house enzyme-linked immunosorbent assay (ELISA), the dot-blot assay, and commercial ELISA.
In-house ELISA, commercial ELISA, and dot-blot test results were positive for anti-AChR antibodies in 65 (76.5%) patients with MG. The results of all 3 tests were negative for anti-AChR antibodies in healthy controls and patients without MG. We observed perfect concordance (K = 1, P <.001) between all 3 tests. In-house ELISA correlated significantly (r = 0.873, P <.001) with commercial ELISA. In-house ELISA and the dot-blot test demonstrated similar diagnostic performance in detecting anti-AChR antibodies.
The dot-blot assay is a simple, nonradioactive immune assay for rapid detection of anti-AChR antibodies in MG.
评估斑点印迹检测在快速、灵敏检测重症肌无力（MG）患者抗乙酰胆碱受体（抗AChR）抗体中的实用性。
在这项病例对照研究中，我们检测了85例MG患者、85名健康对照个体以及85例无MG但患有其他自身免疫性和神经系统疾病患者的血清标本。所有血清标本均采用3种检测方法检测抗AChR抗体：内部酶联免疫吸附测定（ELISA）、斑点印迹测定和商用ELISA。
内部ELISA、商用ELISA和斑点印迹检测结果显示，65例（76.5%）MG患者的抗AChR抗体呈阳性。健康对照和无MG患者的所有3种检测结果中抗AChR抗体均为阴性。我们观察到所有3种检测之间具有完全一致性（K = 1，P <.001）。内部ELISA与商用ELISA显著相关（r = 0.873，P <.001）。内部ELISA和斑点印迹检测在检测抗AChR抗体方面表现出相似的诊断性能。
斑点印迹测定是一种简单、无放射性的免疫测定方法，可用于快速检测MG患者的抗AChR抗体。
