Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.
Mol Med Rep. 2019 Feb;19(2):895-900. doi: 10.3892/mmr.2018.9703. Epub 2018 Nov 28.
Asthma is a common chronic inflammatory respiratory disease characterised by airway inflammation and hyperresponsiveness. The present study was designed to clarify the effect of intranasal miR‑410 administration in an ovalbumin (OVA)‑induced murine model of asthma. It was found that miR‑410 expression was significantly decreased in the lungs of OVA‑induced asthmatic mice (P<0.05) and miR‑410 was overexpressed via intranasal instillation. Bioinformatics indicated that the 3'‑untranslated regions of interleukin (IL)‑4 and IL‑13 messenger RNAs (mRNAs) contain miR‑410 binding sites. The IL‑4 and IL‑13 genes were confirmed to be miR‑410‑regulated using the dual‑luciferase reporter assay. Additionally, intranasal administration of miR‑410 markedly attenuated airway inflammation and reduced infiltration of inflammatory cells into bronchoalveolar lavage fluid (P<0.05) as determined by bronchoalveolar lavage fluid analysis. Moreover, miR‑410 significantly decreased the lung expression of IL‑4 and IL‑13 (P<0.05), although the levels of mRNAs encoding IL‑4 and IL‑13 in lungs did not change significantly as determined by real‑time PCR analysis. In conclusion, we found that intranasal administration of miR‑410 effectively inhibited airway inflammation in OVA‑induced asthmatic mice by targeting IL‑4 and IL‑13 at the post‑transcriptional level. miR‑410 is thus a promising treatment for allergic asthma.
哮喘是一种常见的慢性炎症性呼吸系统疾病，其特征为气道炎症和高反应性。本研究旨在阐明鼻内给予 miR-410 对卵清蛋白（OVA）诱导的哮喘小鼠模型的影响。结果发现，OVA 诱导的哮喘小鼠肺部 miR-410 的表达显著降低（P<0.05），并通过鼻内滴注过表达 miR-410。生物信息学分析表明，白细胞介素（IL）-4 和 IL-13 信使 RNA（mRNA）的 3'非翻译区含有 miR-410 结合位点。使用双荧光素酶报告基因检测证实，IL-4 和 IL-13 基因受 miR-410 调控。此外，通过支气管肺泡灌洗分析，鼻内给予 miR-410 可显著减轻气道炎症并减少炎症细胞浸润到支气管肺泡灌洗液中（P<0.05）。此外，miR-410 显著降低了肺中 IL-4 和 IL-13 的表达（P<0.05），尽管通过实时 PCR 分析并未发现肺中编码 IL-4 和 IL-13 的 mRNAs 水平有明显变化。总之，我们发现鼻内给予 miR-410 通过靶向 IL-4 和 IL-13 发挥作用，可有效抑制 OVA 诱导的哮喘小鼠气道炎症。因此，miR-410 有望成为治疗过敏性哮喘的一种方法。
