The Department of Conservative Dentistry, Medical University of Warsaw, Miodowa 18, 00-246, Warsaw, Poland.
Laboratory of Centre for Preclinical Research, Department of Methodology, Medical University of Warsaw, Banacha 1b, 02-097, Warsaw, Poland.
Histochem Cell Biol. 2019 Nov;152(5):345-353. doi: 10.1007/s00418-019-01811-6. Epub 2019 Sep 5.
The objective of this study is to evaluate MMP-14 expression in odontoblasts and in the bulk of dental pulp of teeth with pulpitis; to determine the expression of microRNA-410 (miR-410) in pulp tissue, since sequence analysis suggests that miR-410 has potential binding site on MMP-14's 3'UTR, and hence, can regulate expression of the latter one. Tissue samples of dental pulp from teeth with pulpitis and healthy (control) were formalin fixed and paraffin embedded (FFPE). Samples were examined using immunohistochemical staining for MMP-14 and the expression of miR-410 was evaluated using qRT-PCR. In both, healthy and inflamed pulp odontoblasts stained more intensively than remaining pulp tissue, but this difference was not statistically significant. More positive staining was observed in inflamed pulps compared to healthy pulps. Expression of miR-410 was found significantly lower in inflamed pulps than in healthy ones. In the two examined zones, odontoblasts and remaining pulp, miR-410 was expressed on a similar level. No statistically significant correlation of miR-410 and MMP-14 expression was found. We showed that inflammation changes the MMP-14 expression in pulp tissue and odontoblasts. This study demonstrates for the first time miR-410 expression in human dental pulp and that expression of this microRNA was downregulated in inflamed dental pulp and odontoblasts.
本研究旨在评估牙髓炎牙齿的成牙本质细胞和牙髓实质中 MMP-14 的表达；检测牙髓组织中 microRNA-410（miR-410）的表达，因为序列分析表明 miR-410 可能在 MMP-14 的 3'UTR 上具有潜在的结合位点，从而可以调节后者的表达。采用福尔马林固定和石蜡包埋（FFPE）的方法固定和包埋牙髓炎和健康（对照）牙齿的牙髓组织样本。使用 MMP-14 的免疫组织化学染色检查样本，并使用 qRT-PCR 评估 miR-410 的表达。在健康和炎症牙髓中，成牙本质细胞的染色比剩余牙髓组织更强烈，但这种差异无统计学意义。与健康牙髓相比，炎症牙髓中观察到更多的阳性染色。与健康牙髓相比，炎症牙髓中 miR-410 的表达明显降低。在这两个检查区域（成牙本质细胞和剩余牙髓），miR-410 的表达水平相似。未发现 miR-410 和 MMP-14 表达之间存在统计学显著相关性。我们表明，炎症改变了牙髓组织和成牙本质细胞中 MMP-14 的表达。本研究首次证明了 miR-410 在人牙髓中的表达，并表明该 microRNA 在炎症性牙髓和成牙本质细胞中表达下调。
