人类端粒酶逆转录酶单核苷酸多态性与体外受精结局的相关性。
Correlation of human telomerase reverse transcriptase single nucleotide polymorphisms with in vitro fertilisation outcomes.
机构信息
Reproductive Medicine Center, Department of Obstetrics and Gynaecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No.107, Yanjiang Xi Road, Guangzhou, 510120, Guangdong, People's Republic of China.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
出版信息
J Assist Reprod Genet. 2019 Mar;36(3):517-527. doi: 10.1007/s10815-018-1379-y. Epub 2018 Dec 10.
PURPOSE
To explore whether it is possible to predict the number and quality of the embryo using a few particular hTERT SNPs.
METHODS
We included 997 Han Chinese women who were genetically unrelated and underwent assisted reproduction using IVF from September 2014 to December 2015. DNA was genotyped by using TaqMan real-time quantitative PCR.
RESULTS
Among the 997 patients, individuals with the CC genotype of rs2075786 had a significantly lower number of good-quality embryos than those with the TT+TC genotypes. Compared with the CT+CC genotype carriers, patients carrying the TT genotype of rs2853677 had a significantly lower number of oocytes retrieved, mature oocytes and available embryos. Among the 750 patients aged ≤ 35 years, individuals with the AA+AG genotypes of rs2853691 had a significantly higher number of good-quality embryos than those with the GG genotype. The haplotype analysis showed that the TTTG (rs2853672/rs2853669/rs2735940/rs2736108) haplotype was more likely to lead to more than three good-quality embryos in patients aged ≤ 35 years.
CONCLUSIONS
Our study suggests that the hTERT SNP is associated with IVF outcomes.
目的
探讨使用少数特定的端粒酶逆转录酶（hTERT）单核苷酸多态性（SNP）是否可以预测胚胎的数量和质量。
方法
我们纳入了 997 名汉族女性，她们在 2014 年 9 月至 2015 年 12 月期间接受了体外受精（IVF）辅助生殖。使用 TaqMan 实时定量 PCR 对 DNA 进行基因分型。
结果
在 997 名患者中，rs2075786 的 CC 基因型个体的优质胚胎数量明显低于 TT+TC 基因型个体。与 CT+CC 基因型携带者相比，rs2853677 的 TT 基因型携带者的获卵数、成熟卵数和可利用胚胎数明显减少。在≤35 岁的 750 名患者中，rs2853691 的 AA+AG 基因型个体的优质胚胎数量明显高于 GG 基因型个体。单倍型分析显示，TTTG（rs2853672/rs2853669/rs2735940/rs2736108）单倍型更可能导致≤35 岁患者有超过三个优质胚胎。
结论
本研究表明，hTERT SNP 与 IVF 结局相关。
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