一种通过尸检组织研究脑相关疾病的完整蛋白质组学工作流程。

A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue.

作者信息

Reis-de-Oliveira Guilherme, Fioramonte Mariana, Martins-de-Souza Daniel

机构信息

Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.

Center for Neurobiology, University of Campinas (UNICAMP), Campinas, Brazil.

出版信息

Methods Mol Biol. 2019;1916:319-328. doi: 10.1007/978-1-4939-8994-2_31.

DOI:10.1007/978-1-4939-8994-2_31

PMID:30535709

Abstract

Here we describe a mass spectrometry-based proteomics workflow to discovery proteins differentially regulated in brains collected postmortem from mental, neurological, or substance abuse disorders (MNS) patients. One way to maximize protein detection is to carry out enrichment of cellular compartments such as the nucleus, mitochondria and cytosol. Subcellular fractionation improves proteome coverage and may shed light on the role of these organelles in the pathophysiology of MNS.

摘要

在此，我们描述了一种基于质谱的蛋白质组学工作流程，用于发现从精神、神经或物质使用障碍（MNS）患者死后采集的大脑中差异调节的蛋白质。最大化蛋白质检测的一种方法是对细胞核、线粒体和细胞质等细胞区室进行富集。亚细胞分级分离可提高蛋白质组覆盖率，并可能揭示这些细胞器在MNS病理生理学中的作用。