Department of Anatomy and Cell Biology, Université de Sherbrooke, Québec, Canada.
FEBS J. 2013 Nov;280(22):5626-34. doi: 10.1111/febs.12502. Epub 2013 Sep 20.
The elucidation of the subcellular distribution of proteins under different conditions is a major challenge in cell biology. This challenge is further complicated by the multicompartmental and dynamic nature of protein localization. To address this issue, quantitative proteomics workflows have been developed to reliably identify the protein complement of whole organelles, as well as for protein assignment to subcellular location and relative protein quantification based on different cell culture conditions. Here, we review quantitative MS-based approaches that combine cellular fractionation with proteomic analysis. The application of these methods to the characterization of organellar composition and to the determination of the dynamic nature of protein complexes is improving our understanding of protein functions and dynamics.
阐明不同条件下蛋白质的亚细胞分布是细胞生物学的主要挑战。由于蛋白质定位的多区室和动态性质，这一挑战更加复杂。为了解决这个问题，已经开发了定量蛋白质组学工作流程，以可靠地鉴定整个细胞器的蛋白质成分，以及根据不同的细胞培养条件将蛋白质分配到亚细胞位置和相对蛋白质定量。在这里，我们回顾了将细胞分级分离与蛋白质组学分析相结合的基于 MS 的定量方法。这些方法在细胞器组成特征分析和蛋白质复合物动态性质测定中的应用，正在提高我们对蛋白质功能和动态的理解。
