Garner Thomas P, Gavathiotis Evripidis
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
Methods Mol Biol. 2019;1877:217-231. doi: 10.1007/978-1-4939-8861-7_15.
Biomolecular nuclear magnetic resonance (NMR) is a powerful and versatile method for studying both protein-protein interactions (PPIs) and protein-small molecule binding. NMR has been used extensively in the investigation of BCL-2 family proteins revealing the structure of key family members, identifying binding partners and interaction sites, and screening small molecule modulators. In this chapter we discuss the application of NMR to identify interaction sites and structure determination of protein-protein and protein-small molecule complexes using two examples.
生物分子核磁共振（NMR）是一种用于研究蛋白质-蛋白质相互作用（PPI）和蛋白质-小分子结合的强大且通用的方法。NMR已广泛应用于BCL-2家族蛋白的研究，揭示关键家族成员的结构，识别结合伴侣和相互作用位点，并筛选小分子调节剂。在本章中，我们通过两个例子讨论NMR在鉴定蛋白质-蛋白质和蛋白质-小分子复合物的相互作用位点及结构测定方面的应用。
