Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, Tianjin, 300457, People's Republic of China.
QingYunTang Biotech (Beijing) Co., Ltd., Beijing, 100176, China.
Appl Biochem Biotechnol. 2019 Jun;188(2):481-490. doi: 10.1007/s12010-018-02936-5. Epub 2018 Dec 10.
This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa. GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80. Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
本研究探讨了热处理对灰树花多糖结构特征和体外抗肿瘤活性的影响。制备了 GFP-4（在 4°C 下提取）、GFP-4-80（在 GFP-4 上 80°C 处理）和 GFP-80（在 80°C 下提取），化学组成分析表明它们的总糖含量均高于 90%，高效凝胶渗透色谱（HPGPC）、离子色谱（IC）和傅里叶变换红外光谱（FTIR）结果表明 GFP-4 在 80°C 热处理后发生降解和变性，MTT 和 JC-1 结果表明 GFP-4 对 HepG2 细胞的体外抑制作用高于 GFP-4-80 和 GFP-80。本研究表明，80°C 热处理灰树花多糖会破坏其结构，减弱其抗肿瘤作用。
