College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Plant Foods Hum Nutr. 2023 Dec;78(4):783-789. doi: 10.1007/s11130-023-01106-1. Epub 2023 Oct 9.
Grifola frondosa, an edible and medicinal resource, is widely used as functional foods worldwide. To explore bioactive compounds against α-glucosidase, human tumor cells and enterovirus 71 (EV71), eight compounds were isolated from G. frondosa by chromatographic column. Among the isolated compounds, heptadecanoic acid, uridine and adenosine exhibited potent inhibition activity against α-glucosidase, ergosterols and ergosterol-5,8-peroxide showed anti-proliferative activity on tumor cells, while ergosterol and methyl linoleate displayed inhibition against the replication of EV71. Also, to our knowledge, this is the first study to report that fatty acids isolated from G. frondosa show potent inhibition against α-glucosidase and EV71. Further molecular docking results revealed that the active compounds in G. frondosa form hydrogen bonding, hydrophobic interactive and π-stacking with the active sites on the surface of α-glucosidase, CASP3 and VP1 proteins, thus promoting the active compounds to combine with the target protein to form a stable complex, thus playing an antagonistic role. Our results could provide a new active compound and mode of action for G. frondosa to treat diabetes, cancer and EV71-infected patients.
灰树花，一种可食用和药用的资源，在世界范围内被广泛用作功能性食品。为了探索针对α-葡萄糖苷酶、人类肿瘤细胞和肠道病毒 71（EV71）的生物活性化合物，从灰树花中通过色谱柱分离得到了八种化合物。在分离得到的化合物中，十七烷酸、尿苷和腺苷对α-葡萄糖苷酶表现出强烈的抑制活性，麦角甾醇和麦角甾醇-5,8-过氧化物对肿瘤细胞表现出增殖抑制活性，而麦角甾醇和亚油酸甲酯对 EV71 的复制显示出抑制作用。此外，据我们所知，这是首次报道从灰树花中分离得到的脂肪酸对α-葡萄糖苷酶和 EV71 具有强烈的抑制作用。进一步的分子对接结果表明，灰树花中的活性化合物与α-葡萄糖苷酶、CASP3 和 VP1 蛋白表面的活性位点形成氢键、疏水相互作用和π-堆积，从而促进活性化合物与靶蛋白结合形成稳定的复合物，从而发挥拮抗作用。我们的研究结果为灰树花治疗糖尿病、癌症和 EV71 感染患者提供了新的活性化合物和作用模式。
