Li Wenwen, Sun Changyue, Yu Songlin, Pu Zhihua, Zhang Penghao, Xu Kexin, Song Zhenqiang, Li Dachao
State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin, China.
Temperature Laboratory, Tianjin Institute of Metrological Supervision Testing, Tianjin, China.
Biomed Microdevices. 2018 Nov 23;20(4):104. doi: 10.1007/s10544-018-0346-9.
This paper proposes a novel fiber attenuated total reflection (ATR) sensor with silver nanoparticles (AgNPs) on the flattened structure based on mid-infrared spectroscopy for detecting low concentration of glucose with high precision. The flattened structure was designed to add the effective optical path length to improve the sensitivity. AgNPs were then deposited on the surface of the flattened area of the fiber via chemical silver mirror reaction for further improving the sensitivity by enhancing the infrared absorption. Combining the AgNPs modified flattened fiber ATR sensor with a CO laser showed a strong mid-infrared glucose absorption, with an enhancement factor of 4.30. The glucose concentration could be obtained by a five-variable partial least-squares model with a root-mean-square error of 4.42 mg/dL, which satisfies clinical requirements. Moreover, the fiber-based technique provides a pretty good method to fabricate miniaturized ATR sensors that are suitable to be integrated into a microfluidic chip for continuous glucose monitoring with high sensitivity.
本文提出了一种新型的基于中红外光谱的光纤衰减全反射（ATR）传感器，该传感器在扁平结构上负载银纳米颗粒（AgNPs），用于高精度检测低浓度葡萄糖。扁平结构的设计增加了有效光程长度，以提高灵敏度。然后通过化学银镜反应将AgNPs沉积在光纤扁平区域的表面，通过增强红外吸收进一步提高灵敏度。将AgNPs修饰的扁平光纤ATR传感器与CO激光器相结合，显示出强烈的中红外葡萄糖吸收，增强因子为4.30。葡萄糖浓度可以通过五变量偏最小二乘模型获得，均方根误差为4.42mg/dL，满足临床要求。此外，基于光纤的技术为制造小型化ATR传感器提供了一种很好的方法，该传感器适合集成到微流控芯片中，用于高灵敏度的连续葡萄糖监测。
