Katsuda Takeshi, Ochiya Takahiro
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan.
Methods Mol Biol. 2019;1905:117-130. doi: 10.1007/978-1-4939-8961-4_11.
Bipotent liver progenitor cells (LPCs) are promising cell sources for cell transplantation therapy in hepatic disorders as well as biliary dysfunctions. Using a cocktail of small molecules, we recently reported a novel approach to generate bipotent LPCs, named chemically induced liver progenitors (CLiPs), from adult rat hepatocytes. In this chapter, we describe a detailed protocol for the induction of rat CLiPs. We first describe the method to isolate primary rat hepatocytes and then describe how to induce CLiPs from the hepatocytes. In addition, we describe methods to induce the generated CLiPs to differentiate into hepatocytes and biliary epithelial cells.
双潜能肝祖细胞（LPCs）是用于肝脏疾病以及胆汁功能障碍细胞移植治疗的有前景的细胞来源。我们最近报道了一种使用小分子混合物从成年大鼠肝细胞中生成双潜能LPCs的新方法，即化学诱导肝祖细胞（CLiPs）。在本章中，我们描述了诱导大鼠CLiPs的详细方案。我们首先描述分离原代大鼠肝细胞的方法，然后描述如何从肝细胞诱导生成CLiPs。此外，我们还描述了诱导生成的CLiPs分化为肝细胞和胆管上皮细胞的方法。
