Xie Ye, Yao Jia, Jin Weilin, Ren Longfei, Li Xun
The First Clinical Medical College, Lanzhou University, Lanzhou, China.
Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province, Lanzhou, China.
Front Cell Dev Biol. 2021 Nov 26;9:765980. doi: 10.3389/fcell.2021.765980. eCollection 2021.
Limited by the poor proliferation and restricted sources of adult hepatocytes, there is an urgent need to find substitutes for proliferation and cultivation of mature hepatocytes for use in disease treatment, drug approval, and toxicity testing. Hepatocyte-like cells (HLCs), which originate from undifferentiated stem cells or modified adult cells, are considered good candidates because of their advantages in terms of cell source and expansion ability. However, the majority of induced HLCs are in an immature state, and their degree of differentiation is heterogeneous, diminishing their usability in basic research and limiting their clinical application. Therefore, various methods have been developed to promote the maturation of HLCs, including chemical approaches, alteration of cell culture systems, and genetic manipulation, to meet the needs of transplantation and model establishment. This review proposes different cell types for the induction of HLCs, and provide a comprehensive overview of various techniques to promote the generation and maturation of HLCs .
由于成人肝细胞增殖能力差且来源有限，迫切需要寻找用于疾病治疗、药物审批和毒性测试的成熟肝细胞增殖与培养替代品。源自未分化干细胞或经过修饰的成体细胞的肝样细胞（HLCs），因其在细胞来源和扩增能力方面的优势而被视为良好的候选者。然而，大多数诱导生成的HLCs处于未成熟状态，其分化程度存在异质性，这降低了它们在基础研究中的可用性，并限制了其临床应用。因此，人们开发了各种方法来促进HLCs的成熟，包括化学方法、改变细胞培养系统和基因操作，以满足移植和模型建立的需求。本综述提出了用于诱导HLCs的不同细胞类型，并全面概述了促进HLCs生成和成熟的各种技术。
