Ayres Laura Silveira, Bos-Mikich Adriana, Frantz Nilo, Arruda Letícia Schmidt, Loss Eloísa da Silveira
Department of Physiology, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Embriology Laboratory, Nilo Frantz Research and Human Reproduction Center, Porto Alegre, RS, Brazil.
Rev Bras Ginecol Obstet. 2018 Dec;40(12):763-770. doi: 10.1055/s-0038-1676037. Epub 2018 Dec 7.
The aim of the present study was to provide a better understanding of the specific action of two follicle-stimulating hormone (FSH) isoforms (β-follitropin and sheep FSH) on the membrane potential of human cumulus cells.
Electrophysiological data were associated with the characteristics of the patient, such as age and cause of infertility. The membrane potential of cumulus cells was recorded with borosilicate microelectrodes filled with KCl (3 M) with tip resistance of 15 to 25 MΩ. Sheep FSH and β-follitropin were topically administered onto the cells after stabilization of the resting potential for at least 5 minutes.
In cumulus cells, the mean resting membrane potential was - 34.02 ± 2.04 mV ( = 14). The mean membrane resistance was 16.5 ± 1.8 MΩ ( = 14). Sheep FSH (4 mUI/mL) and β-follitropin (4 mUI/mL) produced depolarization in the membrane potential 180 and 120 seconds after the administration of the hormone, respectively.
Both FSH isoforms induced similar depolarization patterns, but β-follitropin presented a faster response. A better understanding of the differences of the effects of FSH isoforms on cell membrane potential shall contribute to improve the use of gonadotrophins in fertility treatments.
本研究的目的是更深入地了解两种促卵泡激素（FSH）同工型（β-促卵泡素和绵羊FSH）对人卵丘细胞膜电位的具体作用。
电生理数据与患者的特征相关联，如年龄和不孕原因。使用填充有3M KCl且尖端电阻为15至25MΩ的硼硅酸盐微电极记录卵丘细胞的膜电位。在静息电位稳定至少5分钟后，将绵羊FSH和β-促卵泡素局部施用于细胞。
在卵丘细胞中，平均静息膜电位为-34.02±2.04mV（n = 14）。平均膜电阻为16.5±1.8MΩ（n = 14）。在施用激素后180秒和120秒，绵羊FSH（4mUI/mL）和β-促卵泡素（4mUI/mL）分别使膜电位去极化。
两种FSH同工型均诱导相似的去极化模式，但β-促卵泡素呈现更快的反应。更好地了解FSH同工型对细胞膜电位影响的差异将有助于改善促性腺激素在生育治疗中的应用。
