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大肠杆菌中通过反向RNA对环腺苷酸受体蛋白基因（crp）进行自体调控的体内证据。

Evidence in vivo for autogenous control of the cyclic AMP receptor protein gene (crp) in Escherichia coli by divergent RNA.

作者信息

Okamoto K, Hara S, Bhasin R, Freundlich M

机构信息

Department of Microbiology, Fujita-Gakuen University, Aichi, Japan.

出版信息

J Bacteriol. 1988 Nov;170(11):5076-9. doi: 10.1128/jb.170.11.5076-5079.1988.

DOI:10.1128/jb.170.11.5076-5079.1988
PMID:3053643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211573/
Abstract

Control of crp expression in vivo was studied by using the cloned crp gene. The synthesis of the product of this gene, cyclic AMP (cAMP) receptor protein (CRP), was strongly reduced by exogenous cAMP. This regulation was completely abolished by the inactivation of a divergent promoter located within the crp promoter region. These data are consistent with our in vitro studies (Okamoto and Freundlich, Proc.Natl. Acad. Sci. USA 83:5000-5004, 1986), which showed that crp autoregulation is due to the inhibition of crp transcription by divergent (antisense) RNA produced by cAMP-CRP activation of the divergent promoter.

摘要

利用克隆的crp基因研究了体内crp表达的调控。该基因产物环磷酸腺苷（cAMP）受体蛋白（CRP）的合成受到外源性cAMP的强烈抑制。位于crp启动子区域内的一个反向启动子失活后，这种调控作用完全消失。这些数据与我们的体外研究结果（冈本和弗罗因德利希，《美国国家科学院院刊》83:5000 - 5004，1986年）一致，体外研究表明crp自身调控是由于反向（反义）RNA对crp转录的抑制，而这种反向（反义）RNA是由反向启动子的cAMP - CRP激活产生的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/6be2ac2cca1b/jbacter00189-0086-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/51c673982efc/jbacter00189-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/8aae9997651f/jbacter00189-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/6be2ac2cca1b/jbacter00189-0086-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/51c673982efc/jbacter00189-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/8aae9997651f/jbacter00189-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9612/211573/6be2ac2cca1b/jbacter00189-0086-b.jpg

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Interaction of cAMP receptor protein with the ompA gene, a gene for a major outer membrane protein of Escherichia coli.环磷酸腺苷受体蛋白与ompA基因的相互作用，ompA基因是大肠杆菌一种主要外膜蛋白的编码基因。
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Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli.证明在发散型P3启动子处形成的TyrR蛋白与RNA聚合酶复合物会抑制RNA聚合酶与大肠杆菌aroP基因的主要启动子P1的结合。
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