Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for, Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, Shaanxi Province, P.R. China.
Chemistry. 2019 Feb 1;25(7):1701-1705. doi: 10.1002/chem.201805502. Epub 2019 Jan 8.
Plant microRNAs (miRNAs) are naturally 2'-O-methylated at the 3'-terminal; as a consequence, they cannot be efficiently detected by traditional target-triggered polymerization reactions. Here, a simple but robust enzyme-free sensing strategy was developed for plant miRNA analysis by using dynamic light scattering (DLS) to monitor the crosslinking of gold nanoparticles (AuNPs) amplified by click chemical ligation. Combining the enzyme-free cycling chemical-ligation-mediated signal amplification, and the intrinsic outstanding ability of DLS for discriminating the extremely low level of particle aggregation in a large pool of monodisperse AuNPs, high sensitivity was achieved and as low as 78.6 fm plant miRNA could be easily detected.
植物 microRNAs (miRNAs) 在 3'-末端天然地 2'-O-甲基化；因此，它们不能被传统的基于靶标的聚合反应有效地检测到。在这里，开发了一种简单但强大的无酶传感策略，用于通过动态光散射 (DLS) 监测点击化学连接放大的金纳米粒子 (AuNPs) 的交联来分析植物 miRNA。结合无酶循环化学连接介导的信号放大，以及 DLS 区分大池单分散 AuNPs 中极少量颗粒聚集的固有卓越能力，实现了高灵敏度，能够轻松检测低至 78.6 fm 的植物 miRNA。
