Samsun Carsamba State Hospital, Department of Ophthalmology, Samsun, Turkey.
Ankara Ataturk Education and Research Hospital, Department of Ophthalmology, Ankara, Turkey.
Cont Lens Anterior Eye. 2019 Oct;42(5):492-496. doi: 10.1016/j.clae.2018.11.015. Epub 2018 Dec 8.
To evaluate the role of sex steroid hormone receptors in corneal epithelium in etiopathogenesis of keratoconus (KC).
Thirty patients with KC who were planned for corneal collagen-crosslinking and 20 patients who were planned for excimer laser for refractive errors included in this study. Corneal epitheliums were curated mechanically during surgeries. Right eyes were evaluated immunohistochemically and left eyes were evaluated by quantitative polymerase chain reaction (qPCR) to investigate estrogenα, estrogenβ, progesterone and androgen receptors.
Immunohistochemically, staining for progesterone and androgen receptors did not significantly differ between KC and control groups (p > 0.05). None of the cases had staining for estrogenα and estrogenβ receptors. qPCR showed that mRNA expressions of estrogenα and androgen receptors were significantly higher in the KC group (p < 0.001).
A significantly higher rate of estrogenα and androgen receptor expressions in corneal epithelium from patients with KC through qPCR supports a possible relation between KC and sex steroid hormones.
评估性甾体激素受体在圆锥角膜发病机制中的角膜上皮中的作用。
本研究纳入了 30 名计划行角膜胶原交联术的圆锥角膜患者和 20 名计划行准分子激光屈光不正手术的患者。手术过程中机械性采集角膜上皮。对右眼进行免疫组织化学评估，对左眼进行实时聚合酶链反应（qPCR）评估，以研究雌激素α、雌激素β、孕激素和雄激素受体。
免疫组织化学显示，孕激素和雄激素受体在圆锥角膜组和对照组之间无显著差异（p>0.05）。没有病例显示雌激素α和雌激素β受体染色。qPCR 显示，qPCR 显示，在 KC 组中，雌激素α和雄激素受体的 mRNA 表达明显升高（p<0.001）。
qPCR 显示，KC 患者角膜上皮中雌激素α和雄激素受体的表达率明显升高，这支持 KC 与性甾体激素之间可能存在关系。
