通过血小板淋巴细胞免疫荧光试验（PLIFT）对检测血小板反应性的血清中的抗人白细胞抗原（HLA）抗体进行评估。

Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT).

作者信息

Muylle L, Van Bockstaele D, De Smet D, Peetermans M E

机构信息

Blood Transfusion Center Antwerp, Belgian Red Cross, Edegem.

出版信息

J Immunol Methods. 1988 Nov 10;114(1-2):187-90. doi: 10.1016/0022-1759(88)90172-x.

DOI:10.1016/0022-1759(88)90172-x

PMID:3053909

Abstract

An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.

摘要

本文描述了一种使用血小板单核细胞悬液的间接免疫荧光试验。该试验是一种简单且灵敏的方法，用于评估检测血小板反应性的血清中抗HLA抗体的污染情况，并且无需支持两个独立的检测系统，如淋巴细胞毒性试验和免疫荧光检测。该试验在常规血小板血清学检测和血小板交叉配型中可能具有价值。