Freedman J, Hornstein A
Department of Immunohematology, St. Michael's Hospital, University of Toronto, Canada.
Am J Hematol. 1991 Dec;38(4):314-20. doi: 10.1002/ajh.2830380411.
Since platelets express both platelet-specific and class I HLA antigens, serum antiplatelet reactivity assessed by most platelet antibody techniques could be due to antibodies with either or both specificities. Flow cytometric analysis of sera for detection of antiplatelet antibody commonly employs a purified platelet preparation as target cells. A method is described for investigating sera for platelet antibodies by flow cytometry using a mixture of platelets and lymphocytes. The mixture of lymphocytes and platelets as target cells has the advantage of confirming the presence of the HLA antibodies in reactive sera. The concomitant use of platelets and lymphocytes treated with citric acid, pH3, or with chloroquine (to remove or alter surface HLA antigens without affecting platelet specific antigens) may further assist in identifying antiplatelet antibodies in alloimmunized patients. These techniques may also be useful in platelet crossmatching procedures.
由于血小板表达血小板特异性抗原和I类HLA抗原，大多数血小板抗体技术所评估的血清抗血小板反应性可能归因于具有其中一种特异性或两种特异性的抗体。通过流式细胞术分析血清以检测抗血小板抗体通常采用纯化的血小板制剂作为靶细胞。本文描述了一种使用血小板和淋巴细胞混合物通过流式细胞术研究血清中血小板抗体的方法。将淋巴细胞和血小板的混合物作为靶细胞具有确认反应性血清中HLA抗体存在的优势。同时使用经pH3柠檬酸处理或氯喹处理的血小板和淋巴细胞（以去除或改变表面HLA抗原而不影响血小板特异性抗原）可能有助于进一步鉴定同种免疫患者中的抗血小板抗体。这些技术在血小板交叉配型程序中也可能有用。
