Visani G, Lemoli R M, Dinota A, Cavo M, Gobbi M, Tura S
Institute of Hematology L. & A. Seragnoli, University of Bologna, Italy.
Int J Cell Cloning. 1988 Sep;6(5):313-23. doi: 10.1002/stem.5530060503.
A highly efficient cloning system for in vitro myeloma colony growth could be valuable for screening antineoplastic agents in resistant patients and for testing the effects of purging methods in the context of autologous bone marrow transplantation. In this paper we report the results of experiments intended to improve the myeloma cloning system in plasma clot originally described by Ludwig et al. We tested the effects of the addition of phytohemagglutinin (PHA), coupled with a transformation of the original plasma clot method into a liquid culture system. A statistically higher number of myeloma colonies was observed in the liquid system in the presence of PHA (20 cases, median 84.5 vs. 9.5; p = 0.005), whereas a single variant (either PHA alone or liquid system alone) did not determine any significant growth variation. The increase in the cloning efficiency was evident even in the cases characterized by low bone marrow plasma cell infiltration, suggesting that this method is suitable for the described purposes.
一种用于体外骨髓瘤集落生长的高效克隆系统，对于筛选耐药患者的抗肿瘤药物以及在自体骨髓移植背景下测试净化方法的效果可能具有重要价值。在本文中，我们报告了旨在改进最初由路德维希等人描述的血浆凝块中骨髓瘤克隆系统的实验结果。我们测试了添加植物血凝素（PHA）的效果，并将原始血浆凝块方法转变为液体培养系统。在存在PHA的液体系统中观察到骨髓瘤集落数量在统计学上显著增加（20例，中位数84.5对9.5；p = 0.005），而单一变量（单独使用PHA或单独使用液体系统）并未导致任何显著的生长变化。即使在骨髓浆细胞浸润较低的病例中，克隆效率的提高也很明显，这表明该方法适用于上述目的。
