College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, China.
Environ Monit Assess. 2018 Dec 11;191(1):16. doi: 10.1007/s10661-018-7139-8.
More information is required to improve our understanding of the presence of organic ultraviolet absorbents (UVAs) in organisms and the risks posed to biota. Effective removal of lipids in the determination of UVAs in biological samples is a sample-processing bottleneck. In this study, we optimized a multistep purification method for fish tissue samples extracted using an ultrasonic-assisted extraction method. The purification method involved performing Florisil column chromatography, redissolving the extract in ethanol, and then performing dispersive solid-phase extraction using primary and secondary amine, Florisil, and C silica sorbents. The purified samples were analyzed by gas chromatography-mass spectrometry. The method was effective and reliable, and was used to detect trace concentrations (ng/g) of 12 UVAs in fish tissue samples. The mean recovery range of the UVAs in fish tissue extracts was 65.4-118%, and the method detection limit range was 0.20-2.50 ng/g dw. The validated method was used to analyze 12 UVAs in fish samples from a local supermarket.
需要更多的信息来提高我们对生物体内有机紫外线吸收剂（UVAs）的存在及其对生物群构成的风险的理解。在生物样本中测定 UVAs 时，有效去除脂质是样品处理的瓶颈。在这项研究中，我们优化了一种多步纯化方法，用于提取超声波辅助提取方法的鱼类组织样本。该纯化方法包括使用 Florisil 柱层析、将提取物重新溶解在乙醇中，然后使用一级和二级胺、Florisil 和 C 硅胶吸附剂进行分散固相萃取。使用气相色谱-质谱法对纯化后的样品进行分析。该方法有效可靠，用于检测鱼类组织样品中痕量浓度（ng/g）的 12 种 UVAs。鱼类组织提取物中 UVAs 的平均回收率范围为 65.4-118%，方法检出限范围为 0.20-2.50ng/g dw。验证后的方法用于分析当地超市购买的鱼类样本中的 12 种 UVAs。
