Peng Xianzhi, Jin Jiabin, Wang Chunwei, Ou Weihui, Tang Caiming
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Chromatogr A. 2015 Mar 6;1384:97-106. doi: 10.1016/j.chroma.2015.01.051. Epub 2015 Jan 23.
A sensitive and reliable method was developed for multi-target determination of 13 most widely used organic ultraviolet (UV) absorbents (including UV filters and UV stabilizers) in aquatic organism tissues. The organic UV absorbents were extracted using ultrasonic-assisted extraction, purified via gel permeation chromatography coupled with silica gel column chromatography, and determined by ultra-high performance liquid chromatography-tandem mass spectrometry. Recoveries of the UV absorbents from organism tissues mostly ranged from 70% to 120% from fish filet with satisfactory reproducibility. Method quantification limits were 0.003-1.0ngg(-1) dry weight (dw) except for 2-ethylhexyl 4-methoxycinnamate. This method has been applied to analysis of the UV absorbents in wild and farmed aquatic organisms collected from the Pearl River Estuary, South China. 2-Hydroxy-4-methoxybenzophenone and UV-P were frequently detected in both wild and farmed marine organisms at low ngg(-1)dw. 3-(4-Methylbenzylidene)camphor and most of the benzotriazole UV stabilizers were also frequently detected in maricultured fish. Octocrylene and 2-ethylhexyl 4-methoxycinnamate were not detected in any sample. This work lays basis for in-depth study about bioaccumulation and biomagnification of the UV absorbents in marine environment.
开发了一种灵敏且可靠的方法，用于多目标测定水生生物组织中13种最常用的有机紫外线（UV）吸收剂（包括紫外线过滤剂和紫外线稳定剂）。采用超声辅助萃取法提取有机紫外线吸收剂，通过凝胶渗透色谱法结合硅胶柱色谱法进行纯化，并采用超高效液相色谱-串联质谱法进行测定。从鱼片中回收紫外线吸收剂的回收率大多在70%至120%之间，重现性良好。除4-甲氧基肉桂酸2-乙基己酯外，方法定量限为0.003-1.0ng g(-1)干重（dw）。该方法已应用于分析从中国南方珠江口采集的野生和养殖水生生物中的紫外线吸收剂。在野生和养殖海洋生物中均频繁检测到低浓度（ng g(-1)dw）的2-羟基-4-甲氧基二苯甲酮和UV-P。在海水养殖鱼类中也经常检测到3-(4-甲基亚苄基)樟脑和大多数苯并三唑类紫外线稳定剂。在任何样品中均未检测到奥克立林和4-甲氧基肉桂酸2-乙基己酯。这项工作为深入研究海洋环境中紫外线吸收剂的生物累积和生物放大奠定了基础。
