Fujii Hideki, Watanabe Kentaro, Kono Keiji, Goto Shunsuke, Watanabe Shuhei, Nishi Shinichi
Division of Nephrology and Kidney Center, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
Clin Exp Nephrol. 2019 May;23(5):589-596. doi: 10.1007/s10157-018-1680-1. Epub 2018 Dec 11.
Recent clinical studies have demonstrated that serum fibroblast growth factor 23 (FGF23) levels have a significant association with left ventricular hypertrophy (LVH). Although LVH is commonly seen in hypertensive patients, the association between FGF23, hypertension, and LVH remains unclear. We aimed to examine the changes in serum and intracardiac FGF23 during the progression of hypertension using spontaneously hypertensive rats (SHR).
Male SHR comprised the experimental group (HT group) and Wistar Kyoto rats served as controls. At 10 weeks, urinary and blood biochemical analyses and blood pressure measurements were performed for both the groups. At 18 weeks, the rats were sacrificed: urinary and blood biochemical analyses and real-time PCR were performed.
At 18 weeks, the relative heart weight and serum N-terminal pro-brain natriuretic peptide and aldosterone levels were significantly greater in the HT group. Serum calcium and phosphate levels were significantly lower, while serum FGF23 levels were significantly higher in the HT group compared to the control group. Further analyses showed that the mRNA expression of FGF23 in the heart was significantly increased in the HT group compared to the control group. Both serum FGF23 levels and intracardiac mRNA expression of FGF23 showed significant correlation with the relative heart weight.
During LVH progression, serum and intracardiac FGF23 increased in hypertension. Although it is unclear whether the change in FGF23 is the cause or result of LVH, the interaction between FGF23 and aldosterone may be associated with the development of LVH in hypertension.
近期临床研究表明，血清成纤维细胞生长因子23（FGF23）水平与左心室肥厚（LVH）显著相关。尽管LVH在高血压患者中很常见，但FGF23、高血压和LVH之间的关联仍不清楚。我们旨在利用自发性高血压大鼠（SHR）研究高血压进展过程中血清和心脏内FGF23的变化。
雄性SHR组成实验组（HT组），Wistar Kyoto大鼠作为对照组。10周时，对两组进行尿液和血液生化分析以及血压测量。18周时，处死大鼠：进行尿液和血液生化分析以及实时PCR。
18周时，HT组的相对心脏重量、血清N末端脑钠肽前体和醛固酮水平显著更高。与对照组相比，HT组的血清钙和磷水平显著更低，而血清FGF23水平显著更高。进一步分析表明，与对照组相比，HT组心脏中FGF23的mRNA表达显著增加。血清FGF23水平和心脏内FGF23的mRNA表达均与相对心脏重量显著相关。
在LVH进展过程中，高血压患者的血清和心脏内FGF23升高。虽然尚不清楚FGF23的变化是LVH的原因还是结果，但FGF23与醛固酮之间的相互作用可能与高血压患者LVH的发生有关。
