Noe Remi, Chauvet Sophie, Togarsimalemath Shambhuprasad K, Marinozzi Maria Chiara, Radanova Maria, Vasilev Vasil V, Fremeaux-Bacchi Veronique, Dragon-Durey Marie-Agnes, Roumenina Lubka T
INSERM, UMR_S 1138, Centre de Recherche des Cordeliers, Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, Paris, France.
Methods Mol Biol. 2019;1901:271-280. doi: 10.1007/978-1-4939-8949-2_24.
The innate immune complement system is a powerful defense cascade against pathogens, but can induce host tissue damage when overactivated. In pathological conditions, mainly but not restricted to renal diseases, such as lupus nephritis, atypical hemolytic uremic syndrome, and C3 glomerulopathies, complement is overactivated or dysregulated by autoantibodies directed against its components and regulators. Among the key autoantibody targets are the initiator of the classical complement pathway C1q, the alternative pathway regulator Factor H, the components of the alternative pathway C3 convertase complex C3 and Factor B and the convertase complex itself. This methodological article describes our experience with a method for detection of anti-complement autoantibodies in real time using surface plasmon resonance-based technology. It allows label-free evaluation of the binding efficacy and stability of the formed antigen-antibody complexes.
先天性免疫补体系统是抵御病原体的强大防御级联反应，但过度激活时会诱导宿主组织损伤。在病理状态下，主要但不限于肾脏疾病，如狼疮性肾炎、非典型溶血尿毒综合征和C3肾小球病，补体因针对其成分和调节因子的自身抗体而过度激活或失调。关键的自身抗体靶点包括经典补体途径的启动子C1q、替代途径调节因子H因子、替代途径C3转化酶复合物的成分C3和B因子以及转化酶复合物本身。这篇方法学文章描述了我们使用基于表面等离子体共振技术实时检测抗补体自身抗体的方法的经验。它允许对形成的抗原-抗体复合物的结合效力和稳定性进行无标记评估。
