Xue Rui, Yan Wenchao, Qian Weifeng, Wang Tianqi, Zhang Min, Wei Zhiguo, Han Lifang, He Bin, Dou Jichen
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Acta Trop. 2019 Feb;190:339-343. doi: 10.1016/j.actatropica.2018.12.015. Epub 2018 Dec 10.
Cattle are the intermediate hosts for five Sarcocystis species including S. hominis and S. heydorni, which also infect humans. To investigate the prevalence of Sarcocystis infections in beef products from 17 cities in the Henan Province of central China, 62 raw beef samples from markets were collected and analyzed for Sarcocystis presence via muscle squashing microscopic observation, histological section examination, and molecular characterization with 18S rRNA gene sequencing. Sarcocystis were detected in a total of 20 of the meat samples. Four species were identified that comprised S. cruzi, S. rommeli, S. heydorni, and S. hirsuta, with S. cruzi as the dominant species. In addition, seven of the 20 infected samples were infected with two or three species. Analysis of the 18S rRNA sequences recovered from these samples suggested very little genetic diversity within each species. This study represents the first molecular identification of Sarcocystis species infection in retail beef products from China. These findings will provide valuable information for evaluating the potential public health risk of bovine Sarcocystis species infections and the control of sarcocystosis in cattle.
牛是包括人肉孢子虫和荷氏肉孢子虫在内的5种肉孢子虫的中间宿主，这两种肉孢子虫也会感染人类。为调查中国中部河南省17个城市牛肉产品中肉孢子虫的感染率，采集了62份市售生牛肉样本，通过肌肉压片显微镜观察、组织切片检查以及18S rRNA基因测序进行分子鉴定，以分析肉孢子虫的存在情况。共在20份肉样中检测到肉孢子虫。鉴定出4个种类，包括克鲁兹肉孢子虫、罗梅利肉孢子虫、荷氏肉孢子虫和刚毛刺肉孢子虫，其中克鲁兹肉孢子虫为优势种。此外，在20份受感染样本中，有7份感染了两种或三种肉孢子虫。对从这些样本中获得的18S rRNA序列分析表明，每个种类内的遗传多样性非常低。本研究是对中国市售牛肉产品中肉孢子虫种类感染的首次分子鉴定。这些发现将为评估牛源肉孢子虫感染的潜在公共卫生风险以及控制牛的肉孢子虫病提供有价值的信息。
