Ferreira Maiara S T, Vogel Fernanda Silveira Flores, Sangioni Luis A, Cezar Alfredo Skrebsky, Braunig Patrícia, de Avilla Botton Sônia, Camillo Giovana, Portella Luiza Pires
Laboratório de Doenças Parasitárias, prédio 44, sala 5149, Universidade Federal de Santa Maria (UFSM), Camobi, Santa Maria, RS 97105-900, Brazil.
Laboratório de Doenças Parasitárias, prédio 44, sala 5149, Universidade Federal de Santa Maria (UFSM), Camobi, Santa Maria, RS 97105-900, Brazil.
Vet Parasitol Reg Stud Reports. 2018 Dec;14:94-98. doi: 10.1016/j.vprsr.2018.09.002. Epub 2018 Sep 5.
Sarcocystis spp. are ubiquitous protozoan parasites that can form cysts in striated muscle and CNS of cattle. Cattle hearts are commonly infected by microscopic sarcocysts. Humans can get infected by consuming cattle meat containing the zoonotic parasites Sarcocystis hominis and Sarcocystis heydorni. However, bovine myocardium is generally infected by Sarcocystis cruzi. The aims of this study were to investigate the occurrence of sarcocysts and the identity of Sarcocystis species present in cattle hearts destined to human consumption in the Central region of the Rio Grande do Sul state, southern Brazil. A total of 314 cattle myocardium samples collected from a local abattoir were microscopically examined for the presence of sarcocysts. The sarcocysts isolated from 134 of these samples (ten sarcocysts per sample) were subjected to DNA extraction and PCR amplification. The PCR-amplified DNA fragments were digested with the restriction enzymes BclI and RsaI (PCR-RFLP) for differentiation among S. cruzi, S. hirsuta, and S. hominis. Sarcocystis species identification was confirmed using DNA sequencing of the cox1 mitochondrial DNA. Sarcocysts were detected in all the bovine myocardium samples. PCR-RFLP analysis resulted in successful amplification of 78 of the 134 samples tested. Only the S. cruzi DNA restriction pattern was identified from all of the 78 amplified samples. DNA sequencing also confirmed the presence of S. cruzi DNA. In conclusion, all myocardium samples evaluated were infected with microscopic sarcocysts. S. cruzi was the only species detected infecting the cattle hearts.
肉孢子虫属是普遍存在的原生动物寄生虫，可在牛的横纹肌和中枢神经系统中形成囊肿。牛心脏通常被微小的肉孢子虫囊肿感染。人类通过食用含有动物性寄生虫人肉孢子虫和赖氏肉孢子虫的牛肉而感染。然而，牛心肌通常感染的是克鲁兹肉孢子虫。本研究的目的是调查巴西南部南里奥格兰德州中部地区供人类食用的牛心脏中肉孢子虫囊肿的发生率以及肉孢子虫种类的鉴定。从当地一家屠宰场收集了总共314份牛心肌样本，通过显微镜检查肉孢子虫囊肿的存在情况。从其中134份样本（每份样本10个肉孢子虫）中分离出的肉孢子虫进行DNA提取和PCR扩增。PCR扩增的DNA片段用限制性内切酶BclI和RsaI进行消化（PCR-RFLP），以区分克鲁兹肉孢子虫、多毛肉孢子虫和人肉孢子虫。使用细胞色素c氧化酶亚基1（cox1）线粒体DNA的DNA测序确认肉孢子虫种类鉴定。在所有牛心肌样本中均检测到肉孢子虫囊肿。PCR-RFLP分析在测试的134份样本中有78份成功扩增。在所有78份扩增样本中仅鉴定出克鲁兹肉孢子虫的DNA限制性图谱。DNA测序也证实了克鲁兹肉孢子虫DNA的存在。总之，所有评估的心肌样本均被微小的肉孢子虫囊肿感染。克鲁兹肉孢子虫是检测到的感染牛心脏的唯一物种。
