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基于聚(3,4-亚乙基二氧噻吩)/氧化石墨烯修饰丝网印刷碳电极的克伦特罗高灵敏度免疫传感器的研制。

Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode.

机构信息

Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.

Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.

出版信息

Sensors (Basel). 2018 Dec 7;18(12):4324. doi: 10.3390/s18124324.

Abstract

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R² = 0.9619 and low limit of detection (0.196 ng mL) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

摘要

克伦特罗(CLB)是一种抗生素和非法生长促进药物,具有较长的半衰期,很容易残留并污染动物源性食品,导致各种健康问题。在这项工作中,开发了一种基于聚(3,4-乙二氧基噻吩)/氧化石墨烯(PEDOT/GO)修饰丝网印刷碳电极(SPCE)的电化学免疫传感器,用于食品中抗生素的监测。通过电化学聚合将 SPCE 用 PEDOT/GO 进行修饰,作为传感器平台,而电化学分析则采用直接竞争格式进行,溶液中的游离 CLB 和克伦特罗辣根过氧化物酶(CLB-HRP)将与固定在修饰电极表面的多克隆抗克伦特罗抗体(Ab)竞争结合。报道了具有 R² = 0.9619 的线性标准 CLB 校准曲线和低检测限(0.196ng mL)。对牛奶样品的分析表明,该免疫传感器能够检测实际样品中的 CLB,并且获得的结果与酶联免疫吸附测定(ELISA)相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a00/6308686/94a7213c2414/sensors-18-04324-g001.jpg

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