Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T1Z4, Canada.
Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T1Z4, Canada.
Food Chem. 2018 Apr 25;246:437-441. doi: 10.1016/j.foodchem.2017.12.022. Epub 2017 Dec 7.
In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.
本研究开发了一种基于纸质的微流控酶联免疫吸附测定（ELISA）作为一种快速检测克伦特罗（一种被非法用作食品生产动物生长促进剂的药物）的筛选系统。微流控纸基分析器件（μPAD）与 ELISA 相结合，纸的固有特性允许通过纤维素纤维捕获抗体，这验证了其作为食品安全监测的 96 孔 ELISA 微孔板替代物的潜力。通过测量与分析物浓度成正比的颜色变化强度来检测牛奶中的克伦特罗，检测限为 0.2 ppb。μPAD 有效地降低了用于食品样品检测的 ELISA 的成本、试剂体积和所需时间。
