Trukhan M E, Gorovits R L, Lebedeva M I, Lapidus A L, Mashko S V
Mol Biol (Mosk). 1988 Jul-Aug;22(4):1033-44.
The study on the rate of initiation of model gene cat transcription under the control of E. coli (Plac UV5, Ptrp, Pcat, Ptac), phage lambda (PL, PR), phi X174 (PD) promotors was carried out by means of hybridization of pulse labelled in vivo mRNA with the DNA coding parts. The presence of gene bla(Apr) with its own constitutive promoter in all the recombinants permitted to use the level of appropriate mRNA in the cell as an internal standard. This method allowed to evaluate the true efficiency of the promoters in question. The strength of the promoters studied is shown to be equal within the limit of one order value.
通过体内脉冲标记的mRNA与DNA编码部分的杂交，对在大肠杆菌（Plac UV5、Ptrp、Pcat、Ptac）、噬菌体λ（PL、PR）、φX174（PD）启动子控制下模型基因cat转录起始速率进行了研究。所有重组体中存在带有自身组成型启动子的基因bla(Apr)，这使得能够将细胞中相应mRNA的水平用作内标。该方法能够评估所研究启动子的真实效率。所研究的启动子强度在一个数量级范围内显示为相等。
