• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

表观遗传修饰（乙酰化和去乙酰化）在保幼激素作用中发挥重要作用。

Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.

机构信息

Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546, USA.

Faculty of Forestry and Wood Sciences, EXTEMIT-K, Czech University of Life Sciences, Kamýcká 1176, Prague 6, 165 21, Suchdol, Czech Republic.

出版信息

BMC Genomics. 2018 Dec 14;19(1):934. doi: 10.1186/s12864-018-5323-4.

DOI:10.1186/s12864-018-5323-4
PMID:30547764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6295036/
Abstract

BACKGROUND

Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action.

RESULTS

Exposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment.

CONCLUSIONS

These data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action.

摘要

背景

已知表观遗传修饰（包括 DNA 甲基化和组蛋白的翻译后修饰）可调节基因表达。组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）的拮抗活性介导了果蝇和其他昆虫发育过程中的转录重编程。保幼激素（JH）在生长、发育、变态、生殖和其他生理过程的调控中起着至关重要的作用。然而，我们目前对 JH 作用的表观遗传调控的理解仍然有限。因此，我们研究了 CREB 结合蛋白（CBP，含有 HAT 结构域）和 Trichostatin A（TSA，HDAC 抑制剂）对 JH 作用的影响。

结果

暴露于褐飞虱细胞（TcA 细胞）的 JH 或 TSA 分别导致 Kr-h1（已知的 JH 反应基因）和 31 或 698 个其他基因的表达增加。编码 CBP 的基因敲低导致包括 Kr-h1 在内的 456 个基因的表达减少。有趣的是，几个已知介导 JH 作用的转录因子、核受体、P450 和脂肪酸合酶家族成员的基因的表达受到 CBP 敲低或 TSA 处理的影响。

结论

这些数据表明，HATs 和 HDACs 介导的乙酰化和去乙酰化在 JH 作用中起着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/03930373a1eb/12864_2018_5323_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/b63029080306/12864_2018_5323_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/b9fd8f0d7a7b/12864_2018_5323_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/c935606cdbd9/12864_2018_5323_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/03930373a1eb/12864_2018_5323_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/b63029080306/12864_2018_5323_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/b9fd8f0d7a7b/12864_2018_5323_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/c935606cdbd9/12864_2018_5323_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/6295036/03930373a1eb/12864_2018_5323_Fig6_HTML.jpg

相似文献

1
Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.表观遗传修饰（乙酰化和去乙酰化）在保幼激素作用中发挥重要作用。
BMC Genomics. 2018 Dec 14;19(1):934. doi: 10.1186/s12864-018-5323-4.
2
CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum.CREB 结合蛋白在赤拟谷盗（Tribolium Castaneum）的保幼激素作用中发挥关键作用。
Sci Rep. 2018 Jan 23;8(1):1426. doi: 10.1038/s41598-018-19667-6.
3
Histone deacetylase 1 suppresses Krüppel homolog 1 gene expression and influences juvenile hormone action in .组蛋白去乙酰化酶1抑制克氏同源盒基因1的表达并影响保幼激素的作用。
Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):17759-17764. doi: 10.1073/pnas.1909554116. Epub 2019 Aug 22.
4
Multiple functions of CREB-binding protein during postembryonic development: identification of target genes.CREB 结合蛋白在胚胎后期发育中的多种功能：靶基因的鉴定。
BMC Genomics. 2017 Dec 29;18(1):996. doi: 10.1186/s12864-017-4373-3.
5
CREB-binding protein regulates metamorphosis and compound eye development in the yellow fever mosquito, Aedes aegypti.CREB 结合蛋白调控黄热病蚊，埃及伊蚊的变态和复眼发育。
Biochim Biophys Acta Gene Regul Mech. 2020 Aug;1863(8):194576. doi: 10.1016/j.bbagrm.2020.194576. Epub 2020 May 7.
6
Krüppel homolog 1, an early juvenile hormone-response gene downstream of Methoprene-tolerant, mediates its anti-metamorphic action in the red flour beetle Tribolium castaneum.克虏伯同源物1是耐甲氧普烯下游的一种早期保幼激素反应基因，介导其在赤拟谷盗中的抗变态作用。
Dev Biol. 2009 Jan 15;325(2):341-50. doi: 10.1016/j.ydbio.2008.10.016. Epub 2008 Oct 25.
7
Methoprene-tolerant is essential for embryonic development of the red flour beetle Tribolium castaneum.耐灭蝇胺是赤拟谷盗 Tribolium castaneum 胚胎发育所必需的。
J Insect Physiol. 2020 Feb-Mar;121:104017. doi: 10.1016/j.jinsphys.2020.104017. Epub 2020 Jan 20.
8
Histone deacetylase 3 is required for development and metamorphosis in the red flour beetle, Tribolium castaneum.组蛋白去乙酰化酶 3 在赤拟谷盗（Tribolium castaneum）的发育和变态过程中是必需的。
BMC Genomics. 2020 Jun 22;21(1):420. doi: 10.1186/s12864-020-06840-3.
9
JH modulates a cellular immunity of Tribolium castaneum in a Met-independent manner.JH以一种不依赖Met的方式调节赤拟谷盗的细胞免疫。
J Insect Physiol. 2014 Apr;63:40-7. doi: 10.1016/j.jinsphys.2014.02.008. Epub 2014 Mar 4.
10
N(alpha)-acetyltransferase 40-mediated histone acetylation plays an important role in ecdysone regulation of metamorphosis in the red flour beetle, Tribolium castaneum.N（α）-乙酰转移酶40介导的组蛋白乙酰化在赤拟谷盗（Tribolium castaneum）蜕皮激素调节变态过程中起重要作用。
Commun Biol. 2024 May 3;7(1):521. doi: 10.1038/s42003-024-06212-7.

引用本文的文献

1
Dietary Sodium Butyrate Supplementation Enhances Silkworm Silk Yield by Simultaneously Promoting Larval Growth and Silk Gland Development.日粮添加丁酸钠通过同时促进幼虫生长和丝腺发育提高家蚕产丝量。
Insects. 2025 Jul 24;16(8):761. doi: 10.3390/insects16080761.
2
N-alpha-acetyltransferase 40 modulates ecdysteroid action through chromatin accessibility changes near the promoters of 20-hydroxyecdysone response genes in Tribolium Castaneum TcA cells.N-α-乙酰基转移酶40通过改变赤拟谷盗TcA细胞中20-羟基蜕皮激素反应基因启动子附近的染色质可及性来调节蜕皮激素作用。
Insect Biochem Mol Biol. 2025 Apr;179:104285. doi: 10.1016/j.ibmb.2025.104285. Epub 2025 Feb 20.
3

本文引用的文献

1
Juvenile hormone signaling - a mini review.保幼激素信号转导——一篇小综述。
Insect Sci. 2019 Aug;26(4):600-606. doi: 10.1111/1744-7917.12614. Epub 2018 Jul 18.
2
CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum.CREB 结合蛋白在赤拟谷盗（Tribolium Castaneum）的保幼激素作用中发挥关键作用。
Sci Rep. 2018 Jan 23;8(1):1426. doi: 10.1038/s41598-018-19667-6.
3
Multiple functions of CREB-binding protein during postembryonic development: identification of target genes.
Histone deacetylases synergistically regulate juvenile hormone signaling in the yellow fever mosquito, Aedes aegypti.
组蛋白去乙酰化酶协同调节埃及伊蚊（黄热病蚊子）中的保幼激素信号传导。
Insect Biochem Mol Biol. 2025 Feb;177:104256. doi: 10.1016/j.ibmb.2024.104256. Epub 2024 Dec 30.
4
Chronologically inappropriate morphogenesis () is required for maintenance of larval stages of fall armyworm.胚胎形态发生的时间异常是秋黏虫幼虫期得以维持的必要条件。
Proc Natl Acad Sci U S A. 2024 Dec 3;121(49):e2411286121. doi: 10.1073/pnas.2411286121. Epub 2024 Nov 26.
5
The functions of DNA methyltransferases during the feeding and development of Haemaphysalis longicornis are potentially associated with lysosome pathways.在长角血蜱的摄食和发育过程中，DNA 甲基转移酶的功能可能与溶酶体途径有关。
BMC Genomics. 2024 Nov 19;25(1):1109. doi: 10.1186/s12864-024-11049-9.
6
Harnessing full-text publications for deep insights into C. elegans and Drosophila biomaps.利用全文出版物深入了解秀丽隐杆线虫和果蝇的生物图谱。
BMC Genomics. 2024 Nov 13;25(1):1080. doi: 10.1186/s12864-024-10997-6.
7
N(alpha)-acetyltransferase 40-mediated histone acetylation plays an important role in ecdysone regulation of metamorphosis in the red flour beetle, Tribolium castaneum.N（α）-乙酰转移酶40介导的组蛋白乙酰化在赤拟谷盗（Tribolium castaneum）蜕皮激素调节变态过程中起重要作用。
Commun Biol. 2024 May 3;7(1):521. doi: 10.1038/s42003-024-06212-7.
8
Juvenile hormone regulates silk gene expression by mA RNA methylation.保幼激素通过 mA RNA 甲基化调节丝氨酸基因表达。
Cell Mol Life Sci. 2023 Oct 23;80(11):331. doi: 10.1007/s00018-023-04996-1.
9
Epigenetic weapons in plant-herbivore interactions: Sulforaphane disrupts histone deacetylases, gene expression, and larval development in Spodoptera exigua while the specialist feeder Trichoplusia ni is largely resistant to these effects.植物-草食动物相互作用中的表观遗传武器：萝卜硫素破坏了小菜蛾中的组蛋白去乙酰化酶、基因表达和幼虫发育，而专食性取食者斜纹夜蛾对这些影响则有很大的抗性。
PLoS One. 2023 Oct 19;18(10):e0293075. doi: 10.1371/journal.pone.0293075. eCollection 2023.
10
The Emerging Role of Epigenetics in Metabolism and Endocrinology.表观遗传学在代谢与内分泌学中的新兴作用
Biology (Basel). 2023 Feb 6;12(2):256. doi: 10.3390/biology12020256.
CREB 结合蛋白在胚胎后期发育中的多种功能：靶基因的鉴定。
BMC Genomics. 2017 Dec 29;18(1):996. doi: 10.1186/s12864-017-4373-3.
4
Cap n collar transcription factor regulates multiple genes coding for proteins involved in insecticide detoxification in the red flour beetle, Tribolium castaneum.帽领转录因子调控赤拟谷盗（Tribolium castaneum）中多个编码参与杀虫剂解毒的蛋白质的基因。
Insect Biochem Mol Biol. 2017 Nov;90:43-52. doi: 10.1016/j.ibmb.2017.09.009. Epub 2017 Sep 23.
5
Regulatory functions of trehalose-6-phosphate synthase in the chitin biosynthesis pathway in Tribolium castaneum (Coleoptera: Tenebrionidae) revealed by RNA interference.RNA干扰揭示了赤拟谷盗（鞘翅目：拟步甲科）中6-磷酸海藻糖合酶在几丁质生物合成途径中的调控功能。
Bull Entomol Res. 2018 Jun;108(3):388-399. doi: 10.1017/S000748531700089X. Epub 2017 Sep 18.
6
The Function and Evolution of Nuclear Receptors in Insect Embryonic Development.核受体在昆虫胚胎发育中的功能与演化。
Curr Top Dev Biol. 2017;125:39-70. doi: 10.1016/bs.ctdb.2017.01.003. Epub 2017 Mar 6.
7
FoxO mediates the timing of pupation through regulating ecdysteroid biosynthesis in the red flour beetle, Tribolium castaneum.在赤拟谷盗（Tribolium castaneum）中，FoxO通过调节蜕皮甾类生物合成来介导化蛹时间。
Gen Comp Endocrinol. 2018 Mar 1;258:149-156. doi: 10.1016/j.ygcen.2017.05.012. Epub 2017 May 16.
8
The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm, .FOXO转录因子通过调节家蚕体内保幼激素的降解来控制昆虫的生长和发育。
J Biol Chem. 2017 Jul 14;292(28):11659-11669. doi: 10.1074/jbc.M117.777797. Epub 2017 May 10.
9
Distinct Roles of Met and Interacting Proteins on the Expressions of Family Genes in Brown Planthopper.Met及其相互作用蛋白在褐飞虱家族基因表达中的不同作用
Front Physiol. 2017 Feb 21;8:100. doi: 10.3389/fphys.2017.00100. eCollection 2017.
10
Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis.保幼激素介导的早熟幼虫-成虫变态抑制的分子机制。
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1057-1062. doi: 10.1073/pnas.1615423114. Epub 2017 Jan 17.