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Met及其相互作用蛋白在褐飞虱家族基因表达中的不同作用

Distinct Roles of Met and Interacting Proteins on the Expressions of Family Genes in Brown Planthopper.

作者信息

Lin Xinda, Zhang Ling, Jiang Yanyun

机构信息

Department of Biology, College of Life Sciences, China Jiliang University Hangzhou, China.

出版信息

Front Physiol. 2017 Feb 21;8:100. doi: 10.3389/fphys.2017.00100. eCollection 2017.

DOI:10.3389/fphys.2017.00100
PMID:28270774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5318429/
Abstract

The family genes encode relatively small proteins that are related to olfaction and are regulated by juvenile hormone (JH). The genes modulate various physiological processes, such as behavioral plasticity in the migratory locust and feeding and courtship behaviors in . Therefore, to understand the regulatory mechanism of these physiological processes, it is important to study the expressions of the genes that are regulated by JH signaling. We used quantitative real-time PCR (qRTPCR) to study the role of JH signaling in the regulation of the family genes in the brown planthopper () through the application of Juvenile hormone III (JHIII) and the down-regulation of key genes in the JH signaling pathway. The topical application of JHIII induced the expressions of most of the family genes, and their expressions decreased 2 and 3 days after the JHIII application. Down-regulating the brown planthopper JH receptor NlMethoprene-tolerant (NlMet) and its interacting partners, NlTaiman (NlTai) and Nlß-Ftz-F1 (Nlß-Ftz), through RNAi, exhibited distinct effects on the expressions of the family genes. The down-regulation of and () increased the expressions of the family genes, while the down-regulation of the Met interacting partners and decreased the expressions of most of the family genes. This work advanced our understanding of the molecular function and the regulatory mechanism of JH signaling.

摘要

这些家族基因编码与嗅觉相关的相对较小的蛋白质，并受保幼激素（JH）调控。这些基因调节各种生理过程，如飞蝗的行为可塑性以及[此处原文缺失物种名称]的取食和求偶行为。因此，为了解这些生理过程的调控机制，研究受JH信号调控的这些基因的表达非常重要。我们通过应用保幼激素III（JHIII）以及下调JH信号通路中的关键基因，利用定量实时PCR（qRTPCR）研究JH信号在褐飞虱（Nilaparvata lugens）中对这些家族基因调控的作用。局部施用JHIII诱导了大多数家族基因的表达，并且在施用JHIII后2天和3天其表达下降。通过RNA干扰下调褐飞虱JH受体耐甲氧普烯（NlMet）及其相互作用伙伴NlTaiman（NlTai）和Nlß-Ftz-F1（Nlß-Ftz），对家族基因的表达表现出不同的影响。下调[此处原文缺失基因名称]和[此处原文缺失基因名称]（[此处原文缺失物种名称]）增加了家族基因的表达，而下调Met相互作用伙伴[此处原文缺失基因名称]和[此处原文缺失基因名称]则降低了大多数家族基因的表达。这项工作增进了我们对JH信号分子功能和调控机制的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/190ba81187ed/fphys-08-00100-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/52bf1a7b70df/fphys-08-00100-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/29c279cede4d/fphys-08-00100-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/42f8832b2709/fphys-08-00100-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/84b2b10e3dce/fphys-08-00100-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/190ba81187ed/fphys-08-00100-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/52bf1a7b70df/fphys-08-00100-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/29c279cede4d/fphys-08-00100-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/42f8832b2709/fphys-08-00100-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/84b2b10e3dce/fphys-08-00100-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5a4/5318429/190ba81187ed/fphys-08-00100-g0005.jpg

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本文引用的文献

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Int J Biol Sci. 2016 Mar 30;12(5):607-16. doi: 10.7150/ijbs.14802. eCollection 2016.
2
JNK signaling mediates wing form polymorphism in brown planthoppers (Nilaparvata lugens).JNK信号传导介导褐飞虱（Nilaparvata lugens）的翅型多态性。
Insect Biochem Mol Biol. 2016 Jun;73:55-61. doi: 10.1016/j.ibmb.2016.04.005. Epub 2016 Apr 24.
3
FOXO links wing form polyphenism and wound healing in the brown planthopper, Nilaparvata lugens.
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4
Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.表观遗传修饰（乙酰化和去乙酰化）在保幼激素作用中发挥重要作用。
BMC Genomics. 2018 Dec 14;19(1):934. doi: 10.1186/s12864-018-5323-4.
5
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6
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